Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.11/942
Título: Isoenzymatic variability in populations of Lupinus from Beira Interior
Autor: Frederico, A.M.
Reis, C.M.G.
Palavras-chave: Lupinus luteus
Isoenzyme polimorphism
Lupinus hispanicus
Data: 2002
Editora: SPB
Citação: FREDERICO, A.M. ; REIS, C.M.G. (2002) - Isoenzymatic variability in populations of Lupinus from Beira Interior. In Congresso Nacional de Bioquímica, 13, Lisboa, 5-7 Dez. - Actas.
Resumo: Isoenzyrnatic studies were performed in order to study the varia¬bility of Lupinus spp. populations from Beira Interior region. Thirty accessions of Lupinus (L. luteus, L. albus, L. angustifoius and L. Hispanicus) from Beira interior region were studied. The zymograms were obtained, by poliacrilamide gel ele¬ctrophoresis (PAGE). Extracts were prepared from young leaf and cotyledons tissues. Four enzyme systems were studied: aspartate aminotransferase (AAT), esterase (EST), acid phos¬phatase (ACT) and malate desidrogenase (MDH). Isoenzyme polymorphisms were found for all the enzyme sys¬tems studied. The comparative analysis of AAT and MDH zymograms show imerspecific variation and made it possible to identify the four species studied. Significant intraspecific varia¬tion was found in EST and ACP zymograms; however intrapopu¬lational variation was also detected and the different popula¬tions could not be distinguished inside each species. The existance of a greater genetic proximity between L. luteus and L. hispanicus was detected.
URI: http://hdl.handle.net/10400.11/942
Aparece nas colecções:ESACB - Posters em encontros científicos/técnicos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Isolation and characterization.pdf254,6 kBAdobe PDFVer/Abrir

FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.