Browsing by Author "Machado, Ana"
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- Analysis of sequence variability of prion protein gene (PRNP) in Portuguese cervid populations species: red deer, fallow deer and roe deerPublication . Pereira, Jorge C.; Machado, Ana; Gonçalves-Anjo, Nuno; Bastos, Estela; Orge, Leonor; Rocha, Sara; Figueira, Luís; Matos, Ana Cristina; Silva, João; Mendonça, Paula; Carvalho, Paulo; Tavares, Paula; Lima, Carla; Alves, Anabela; Esteves, Alexandra; Pinto, Maria de Lurdes; Pires, Isabel; Gama, Adelina; Sargo, Roberto; Silva, Filipe; Seixas, Fernanda; Pinto, Madalena Vieira; Pires, Maria dos AnjosTransmissible Spongiform Encephalopathy (TSE) or prion diseases are a family of neurodegenerative diseases caused by lethal infectious pathogens called Prions. Among the transmissible spongiform encephalopathies (TSEs), chronic wasting disease (CWD) in cervids is now the rising concern in wildlife within Europe, after the first case was detected in Norway in 2016, in a wild reindeer and until October 2021, a total of 40 cases were described in Norway, Sweden and Finland. The study of the genetics of the prion protein gene, PRNP, has been proved to be a valuable tool for determining the relative susceptibility to TSEs.
- Are Western Iberian roe deer populations hotspots of genetic diversity?Publication . Pereira, Jorge C.; Machado, Ana; Saianda, Mafalda; Gonçalves-Anjo, Nuno; Bastos, Estela; Orge, Leonor; Rocha, Sara; Figueira, Luís; Matos, Ana Cristina; Silva, João; Mendonça, Paula; Carvalho, Paulo; Tavares, Paula; Lima, Carla; Alves, Anabela; Esteves, Alexandra; Pinto, Maria de Lurdes; Pires, Isabel; Gama, Adelina; Sargo, Roberto; Silva, Filipe; Seixas, Fernanda; Pinto, Madalena Vieira; Pires, Maria dos AnjosGiven its geographic isolation, and after the last glaciation, the Iberian Peninsula, become a sanctuary of plants and animal’s diversification, being considered a hotspot of genetic diversity specially for mammal species. The roe deer (Capreolus capreolus - CCR), an herbivore ungulate, has a widespread geographical distribution which extends from the Iberian Peninsula to the north of Scandinavia, being found as well in Turkey, Israel, and Jordan. C. capreolus populations have been subject to a significant number of fluctuations, especially due to anthropogenic activities, which led to a decrease in size and distribution. These alterations combined with translocations of animals of the same species, can lead to meaningful consequences on the genetic structure, diversity, and fitness of populations.
- Chronic wasting disease risk assessment in Portugal: analysis of variability and genetic structure of the portuguese roe deer populatioPublication . Gonçalves-Anjo, Nuno; Pereira, Jorge C.; Machado, Ana; Saianda, Mafalda; Bastos, Estela; Orge, Leonor; Rocha, Sara; Figueira, Luís; Matos, Ana Cristina; Silva, João; Mendonça, Paula; Carvalho, Paulo; Tavares, Paula; Lima, Carla; Alves, Anabela; Esteves, Alexandra; Pinto, Maria de Lurdes; Pires, Isabel; Gama, Adelina; Sargo, Roberto; Silva, Filipe; Seixas, Fernanda; Pinto, Madalena Vieira; Pires, Maria dos AnjosAmong the Transmissible Spongiform Encephalopathies, Chronic Wasting Disease (CWD) in cervids is now the rising concern in wildlife within Europe after the first case detected in Norway in 2016. CWD shows a notable horizontal transmission, affecting both free-ranging and captive cervids. Furthermore, several genetic variants in the Prion Protein (PRNP) gene coding sequence of the cervid were identified, which increase the susceptibility to the disease. Roe deer has a widespread geographical distribution which extends from the Iberian Peninsula to the north of Scandinavia, being found as well in Turkey, Israel, and Jordan. In recent centuries, a significant number of fluctuations have occurred in these deer populations, leading to a decline in deer population and distribution. These alterations combined with translocations of animals of the same species, can drive to meaningful consequences on the genetic structure, diversity, and fitness of populations.
- Chronic wasting disease risk assessment in Portugal: analysis of variability and genetic structure of the Portuguese roe deer populationPublication . Gonçalves-Anjo, Nuno; Pereira, Jorge C.; Machado, Ana; Saianda, Mafalda; Bastos, Estela; Matos, Ana Cristina; Gama, Adelina; Alves, Anabela; Esteves, Alexandra; Rocha, Sara; Figueira, Luís; Lima, Carla; Silva, Filipe; Seixas, Fernanda; Pires, Isabel; Silva, João; Pinto, Madalena Vieira; Pinto, Maria de Lurdes; Mendonça, Paula; Carvalho, Paulo; Tavares, Paula; Sargo, Roberto; Orge, Leonor; Pires, Maria dos AnjosAmong the Transmissible Spongiform Encephalopathies, Chronic Wasting Disease (CWD) in cervids is now the rising concern in wildlife within Europe after the first case detected in Norway in 2016. CWD shows a notable horizontal transmission, affecting both free-ranging and captive cervids. Furthermore, several genetic variants in the Prion Protein (PRNP) gene coding sequence of the cervid were identified, which increase the susceptibility to the disease.
- Determining prion protein gene (PRNP) genetic variability in portuguese cervidae population: an important task in chronic wasting disease (CWD) risk assessment projet in PortugalPublication . Pereira, Jorge C.; Machado, Ana; Saianda, Mafalda; Gonçalves-Anjo, Nuno; Bastos, Estela; Orge, Leonor; Rocha, Sara; Figueira, Luís; Matos, Ana Cristina; Silva, João; Mendonça, Paula; Carvalho, Paulo; Tavares, Paula; Lima, Carla; Alves, Anabela; Esteves, Alexandra; Pinto, Maria de Lurdes; Pires, Isabel; Gama, Adelina; Sargo, Roberto; Silva, Filipe; Seixas, Fernanda; Pinto, Madalena Vieira; Pires, Maria dos AnjosThe chronic wasting disease (CWD) in cervids is now a rising concern in wildlife within Europe, since the first case detected in Norway in 2016, 40 more appear until May of 2022, in Norway, Sweden and Finland. The unclear origin of these new European cases and the risk that CWD poses to cohabiting ani-mals or more importantly to humans is largely unknown, is very important for the establishment of risk assessment projects, even in countries with no cases of CWD to forecast possible infections. In this way, a synergistic collaborative project was established between the UTAD, INIAV and IPCB to evaluate the risk of a potential occurrence of CWD in cervid Portuguese populations. The study of prion protein gene, PRNP, has been proved to be a valuable tool for determining the relative susceptibility to TSEs since this is influenced by polymorphisms in this gene. The aim of this work is the screening for PrPresand determina-tion of the PRNP genotyping profile on Portuguese cervids, as the survey and georeferencing of these ani-mals will contribute to delineating the risk of dissemi-nation of CWD in Portugal.
- Physicochemical fingerprint of "Pera Rocha do Oeste". A PDO pear native from PortugalPublication . Pedro, Soraia; Coelho, Elisabete; Peres, Maria de Fátima; Machado, Ana; Rodrigues, A.M.; Wessel, Dulcineia F.; Coimbra, Manuel A.; Anjos, O."Pera Rocha do Oeste" is a pear (Pyrus communis L.) variety native from Portugal with a Protected Designation of Origin (PDO). To supply the world market for almost all the year, the fruits are kept under controlled storage. This study aims to identify which classical physicochemical parameters (colour, total soluble solids (TSS), pH, acidity, ripening index, firmness, vitamin C, total phenols, protein, lipids, fibre, ash, other compounds including carbohydrates, and energy) could be fingerprint markers of PDO "Pera Rocha do Oeste". For this purpose, a data set constituting fruits from the same size, harvested from three orchards of the most representative PDO locations and stored in refrigerated conditions for 2 or 5 months at atmospheric conditions or for 5 months under a modified atmosphere, were selected. To validate the fingerprint parameters selected with the first set, an external data set was used with pears from five PDO orchards stored under different refrigerated conditions. Near infrared (NIR) spectroscopy was used as a complementary tool to assess the global variability of the samples. The lightness of the pulp; the b* CIELab coordinate of the pulp and peel; and the pulp TSS, pH, firmness, and total phenols, due to their lower variability, are proposed as fingerprint markers of this pear.