Browsing by Author "Rosado, Tiago"
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- Caracterização do perfil fenólico em extratos de flores de acácia spp.Publication . Pedro, Soraia; Rosado, Tiago; Barroca, Celina; Neiva, Duarte; Gominho, Jorge; Gallardo, Eugenia; Anjos, O.Caracterização do perfil fenólico em extratos de flores de acácia spp.
- Characterisation of the phenolic profile of Acacia retinodes and Acacia mearnsii flowers’ extractsPublication . Pedro, Soraia; Rosado, Tiago; Barroca, Celina; Neiva, Duarte; Alonso-Herranz, Vanesa; Gradillas, Ana; García, Antonia; Gominho, Jorge; Gallardo, Eugenia; Anjos, O.Acacia spp. is an invasive species that is widespread throughout the Portuguese territory. Thus, it is pertinent to better understand this species in order to find different applications that will value its use. To evaluate the phenolic profile in Acacia flowers, ethanolic extracts obtained through an energized guided dispersive extraction were analysed, focusing on two species, Acacia retinodes and Acacia mearnsii, at two flowering stages. The phytochemical profile of each extract was determined by ultra-high performance liquid chromatography coupled with quadrupole time-offlight mass spectrometry and high-performance liquid chromatography coupled with diode array detector. The FTIR-ATR technique was used to distinguish the different samples’ compositions. The results showed the presence of high concentrations of phenolic compounds (>300 mg GAE/g extract), among which are flavonoids (>136 mg QE/g extract), for all combinations of species/flowering stages. The phytochemical profile showed a complex composition with 21 compounds identified and quantified (the predominant ones being epicatechin, rutin, vanillin, and catechol). Both species and flowering stages presented significant variations regarding the presence and quantity of phenols and flavonoids, so much so that a principal component analysis performed with FTIR-ATR spectra data of the extracts was able to discriminate between species and flowering stages.
- Determinação de canabinóides em amostras de urina por microextração em seringa empacotada e cromatografia gasosa-espetrometria de massaPublication . Rosendo, Luana M.; Rosado, Tiago; Oliveira, P.; Simão, Ana Y.; Antunes, Carlos Alberto Lopes; Anjos, O.; Margalho, C.; Costa, S.; Passarinha, Luís A.; Barroso, M.; Gallardo, EugeniaA canábis é a substância ilícita mais frequentemente consumida a nível mundial. Em laboratórios de análise de drogas. A urina é uma das amostras mais utilizadas para a deteção de canabinóides.
- Insights into the bioactivities and chemical analysis of Ailanthus altissima (Mill.) SwinglePublication . Caramelo, Débora; Pedro, Soraia; Marques, Hernâni; Simão, Ana Y.; Rosado, Tiago; Barroca, Celina; Gominho, Jorge; Anjos, O.; Gallardo, EugeniaMany species of the so-called exotic plants coexist with native species in a balanced way, but others thrive very quickly and escape human control, becoming harmful—these are called invasive alien species. In addition to overcoming geographic barriers, these species can defeat biotic and abiotic barriers, maintaining stable populations. Ailanthus altissima is no exception; it is disseminated worldwide and is considered high risk due to its easy propagation and resistance to external environmental factors. Currently, it has no particular use other than ornamental, even though it is used to treat epilepsy, diarrhea, asthma, ophthalmic diseases, and seborrhoea in Chinese medicine. Considering its rich composition in alkaloids, terpenoids, sterols, and flavonoids, doubtlessly, its use in medicine or other fields can be maximised. This review will focus on the knowledge of the chemical composition and the discovery of the biological properties of A. altissima to understand this plant better and maximise its possible use for purposes such as medicine, pharmacy, or the food industry. Methods for the extraction and detection to know the chemical composition will also be discussed in detail.
- Insights into the bioactivities and chemical analysis of Ailanthus altissima (Mill.) swinglePublication . Caramelo, Débora; Pedro, Soraia; Marques, Hernâni; Simão, Ana Y.; Rosado, Tiago; Barroca, Celina; Gominho, Jorge; Anjos, O.; Gallardo, EugeniaIn recent years, several analytical methods for characterising the Ailanthus altissima (Mill.) Swingle have aroused interest in the scientific community, since this species is not only considered an invasive alien species, but also possesses a wide and complex number of chemical compounds. These compounds are studied with the aim of ascertaining their biological activities, which could help to understand their mechanisms of action, develop new products with potential application in different fields of research.
- Miniaturized solid phase extraction techniques applied to natural productsPublication . Rosendo, Luana M.; Brinca, Ana Teresa; Pires, Bruno; Catarro, Gonçalo; Rosado, Tiago; Guiné, Raquel P.F.; Araújo, André; Anjos, O.; Gallardo, EugeniaNatural products are increasingly becoming part of our daily lives through their use in industry, food, as therapeutic agents, etc. To evaluate their possible applications, it is essential to characterize them chemically to explore their potential. Different techniques may be used to characterize natural products, including microextraction techniques. These techniques have been gaining popularity due to the advantages associated with their low use of organic solvents and the small amount of sample used relative to more classical sample preparation techniques. Their application in the extraction of compounds from natural products is still scarce. This manuscript intends to review the most used solid-based miniaturized sample preparation techniques applied to determining compounds in natural products. The main applications of these methodologies will be discussed, with a particular focus on natural product analysis, as well as their advantages and disadvantages over traditionally used sample preparation techniques.
- Miniaturized solid phase extraction techniques applied to natural productsPublication . Rosendo, Luana M.; Brinca, Ana Teresa; Pires, Bruno; Catarro, Gonçalo; Rosado, Tiago; Martinho, J.P.; Guiné, Raquel P.F.; Araújo, André; Anjos, O.; Galhardo, EugeniaNatural products are increasingly becoming part of our daily lives through their use in industry, food, as therapeutic agents, etc. Different techniques may be used to characterize natural products, including microextraction techniques. This work intends to review the most used solid-based miniaturized sample preparation techniques applied to determining compounds in natural products. Advantages and drawbacks are also presented. A