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Association between a case study of asymptomatic ovine listerial mastitis and the contamination of soft cheese and cheese processing environment with listeria monocytogenes in portugal

2009Journal article Restricted access
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dc.contributor.authorPintado, Cristina Santos
dc.contributor.authorGrant, Kathie
dc.contributor.authorHalford-Maw, Robin
dc.contributor.authorHampton, Mike D.
dc.contributor.authorFerreira, Maria A.S.S.
dc.contributor.authorMcLauchlin, Jim
dc.date.accessioned2022-02-08T12:43:26Z
dc.date.available2022-02-08T12:43:26Z
dc.date.issued2009
dc.date.updated2022-02-07T01:39:08Z
dc.description.abstractFor 5 months, the udders of milking ewes, raw ewe’s milk, cheese, and the plant and environment of a cheese manufacturer in Portugal were investigated using standard methods for the presence of Listeria spp. An association between subclinical mastitis and Listeria monocytogenes in a single lactating sheep was investigated by visual inspection of udders for signs of inflammation, application of somatic cell counts, the California mastitis test, pH measurement to milk, and culture of L. monocytogenes and Staphylococcus spp. To track the routes of contamination by L. monocytogenes, 103 isolates were characterized by molecular serotyping and amplified fragment length polymorphism, and a selection was further tested by pulsed-field gel electrophoresis. This study provides molecular and epidemiological evidence tracking the persistence of a single L. monocytogenes strain causing a subclinical udder infection without obvious inflammation in a single ewe. This infection was the likely source of contamination of raw milk that was subsequently used to produce unpasteurised milk cheese and resulted in a single strain of this bacterium colonizing the processing environment and the final cheese product.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1089/fpd.2008.0246pt_PT
dc.identifier.slugcv-prod-2597191
dc.identifier.urihttp://hdl.handle.net/10400.11/7884
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectOvine listerial mastitispt_PT
dc.subjectListeria monocytogenspt_PT
dc.titleAssociation between a case study of asymptomatic ovine listerial mastitis and the contamination of soft cheese and cheese processing environment with listeria monocytogenes in portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage575pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage569pt_PT
oaire.citation.titleFoodborne Pathogens and Diseasept_PT
oaire.citation.volume6pt_PT
person.familyNameSantos Pintado
person.givenNameCristina Maria Baptista
person.identifier.ciencia-id7F1F-E7E1-5BC6
person.identifier.orcid0000-0001-5433-4258
person.identifier.scopus-author-id6602633775
rcaap.cv.cienciaid7F1F-E7E1-5BC6 | Cristina Maria Baptista Santos Pintado
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione5822f39-6e57-4619-b731-b51b3026cdfe
relation.isAuthorOfPublication.latestForDiscoverye5822f39-6e57-4619-b731-b51b3026cdfe

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