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Variability within a clonal population of Erwinia amylovora disclosed by phenotypic analysis

2022Journal article Open access
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dc.contributor.authorMendes, Rafael J.
dc.contributor.authorSilva, M.C.A.
dc.contributor.authorLuz, J.P.
dc.contributor.authorTavares, Fernando
dc.contributor.authorSantos, Conceição
dc.date.accessioned2023-03-28T15:13:14Z
dc.date.available2023-03-28T15:13:14Z
dc.date.issued2022
dc.description.abstractBackground Fire blight is a destructive disease of pome trees, caused by Erwinia amylovora, leading to high losses of chain-of-values fruits. Major outbreaks were registered between 2010 and 2017 in Portugal, and the first molecular epidemiological characterization of those isolates disclosed a clonal population with different levels of virulence and susceptibility to antimicrobial peptides. Methods This work aimed to further disclose the genetic characterization and unveil the phenotypic diversity of this E. amylovora population, resorting to MLSA, growth kinetics, biochemical characterization, and antibiotic susceptibility. Results While MLSA further confirmed the genetic clonality of those isolates, several phenotypic differences were recorded regarding their growth, carbon sources preferences, and chemical susceptibility to several antibiotics, disclosing a heterogeneous population. Principal component analysis regarding the phenotypic traits allows to separate the strains Ea 630 and Ea 680 from the remaining. Discussion Regardless the genetic clonality of these E. amylovora strains isolated from fire blight outbreaks, the phenotypic characterization evidenced a population diversity beyond the genotype clonality inferred by MLSA and CRISPR, suggesting that distinct sources or environmental adaptations of this pathogen may have occurred. Conclusion Attending the characteristic clonality of E. amylovora species, the data gathered here emphasizes the importance of phenotypic assessment of E. amylovora isolates to better understand their epidemiological behavior, namely by improving source tracking, make risk assessment analysis, and determine strain-specific environmental adaptations, that might ultimately lead to prevent new outbreaks.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMENDES, R.J. [et al.] (2022) - Variability within a clonal population of Erwinia amylovora disclosed by phenotypic analysis. PeerJ. 10:e13695. DOI https://doi.org/10.7717/peerj.13695pt_PT
dc.identifier.doi10.7717/peerj.13695pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.11/8447
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPeerJpt_PT
dc.relationFCT - UIDB/50006/2020 — UIDP/50006/2020pt_PT
dc.relationPearFire: Functional omics of Erwinia-PearRocha pathosystem to characterize virulent strains and resistant pear genotypes
dc.titleVariability within a clonal population of Erwinia amylovora disclosed by phenotypic analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePearFire: Functional omics of Erwinia-PearRocha pathosystem to characterize virulent strains and resistant pear genotypes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F133519%2F2017/PT
oaire.citation.startPagee13695pt_PT
oaire.citation.titlePeerJpt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamPOR_NORTE
person.familyNameMendes
person.familyNameAmaro
person.familyNameLuz
person.familyNameDos Santos
person.givenNameRafael
person.givenNameConceição
person.givenNameJoão Pedro
person.givenNameConceição Domingos José
person.identifier.ciencia-idA712-701F-0EEC
person.identifier.ciencia-idCF19-F43F-86DE
person.identifier.ciencia-idA61A-A0F5-77F8
person.identifier.orcid0000-0001-5212-6172
person.identifier.orcid0000-0002-9642-9519
person.identifier.orcid0000-0002-3494-737X
person.identifier.ridM-7245-2013
person.identifier.scopus-author-id57191629737
person.identifier.scopus-author-id36504399900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7cb85c28-f773-48e3-9e1c-6ffd0f310aef
relation.isAuthorOfPublication2755901d-a3c6-4060-975b-82122772679e
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