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Stevia rebaudiana germplasm characterization using microsatellite markers and steviol glycosides quantification by HPLC

2021Journal article Open access
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dc.contributor.authorRibeiro, M.M.A.
dc.contributor.authorDiamantino, Tatiana
dc.contributor.authorDomingues, Joana Lopes
dc.contributor.authorMontanari, Ílio
dc.contributor.authorAlves, Marcos Nopper
dc.contributor.authorGonçalves, José Carlos
dc.date.accessioned2021-04-23T11:45:19Z
dc.date.available2021-04-23T11:45:19Z
dc.date.issued2021
dc.description.abstractStevia rebaudiana Bertoni, Asteraceae, is an herbaceous perennial plant native to Paraguay. This species is considered since ancient times a medicinal plant with important bioactive compounds and pharmacologic and food properties, namely diterpenes glycosides. The high natural sweetener potential stevioside and rebaudioside A produced by S. rebaudiana plants are suitable sucrose substitutes, and their obtention is influenced by environmental, phytosociological, and genetic factors. The plants' genetic profile and sweet potential depiction are needed for suitable plant selection for improvement and deployment. Thirty-one S. rebaudiana accessions grown in the same plot where leaves samples were collected in early 2019, were genotyped using six microsatellite markers, including two steviol glycosides biosynthesis functionally involved markers. Additionally, an aqueous extract of each sample was obtained in a water bath and purified by SPE for stevioside and rebaudioside A quantification by normal phase HPLC. Stevioside and rebaudioside A contents varied between 0.53-7.36% (w w-1) and 0.37-3.60% (w w-1), respectively. Two genotypes displayed interesting ratios of rebaudioside A/stevioside (number 3 and 33). The level of genetic similarity between genotypes was tested through a pairwise similarity coefficient, and two groups of individuals had the same fingerprinting. Strong relatedness was found within genotypes, possibly due to cloning, thus, influx of new germplasm ought to be made to prevent mating between relatives, and for further selection and genetic improvement.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s11033-021-06308-xpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.11/7520
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationForest Research Centre
dc.relationResearch Center in Natural Resources, Environment and Society
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectFingerprintpt_PT
dc.subjectGenetic improvementpt_PT
dc.subjectGermplasmpt_PT
dc.subjectSteviosidept_PT
dc.subjectMolecular markerspt_PT
dc.subjectRebaudioside Apt_PT
dc.titleStevia rebaudiana germplasm characterization using microsatellite markers and steviol glycosides quantification by HPLCpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleForest Research Centre
oaire.awardTitleResearch Center in Natural Resources, Environment and Society
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00239%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00681%2F2020/PT
oaire.citation.endPage2582pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage2573pt_PT
oaire.citation.titleMolecular Biology Reportspt_PT
oaire.citation.volume48pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameRibeiro
person.familyNameGonçalves
person.givenNameMaria Margarida
person.givenNameJosé
person.identifier349097
person.identifier508085
person.identifier.ciencia-idAD12-4D32-7A48
person.identifier.ciencia-idAE1F-12BE-65DD
person.identifier.orcid0000-0003-4684-1262
person.identifier.orcid0000-0003-3387-4487
person.identifier.ridM-4235-2013
person.identifier.scopus-author-id7201715611
person.identifier.scopus-author-id7103325479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isProjectOfPublication174bc925-4708-4d2c-a7b4-0e5ea68d0d19
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