Publication
Apparent dominance of the G1-G3 genetic cluster of echinococcus granulosus strains in the central inland region of Portugal
| dc.contributor.author | Beato, Sílvia | |
| dc.contributor.author | Parreira, Ricardo | |
| dc.contributor.author | Calado, Manuela | |
| dc.contributor.author | Grácio, Maria Amélia | |
| dc.date.accessioned | 2012-12-17T12:01:16Z | |
| dc.date.available | 2012-12-17T12:01:16Z | |
| dc.date.issued | 2010 | |
| dc.date.updated | 2011-03-04T15:21:16Z | |
| dc.description.abstract | Infection by the larval stage of the cestode Echinococcus granulosus causes a disease known as cystic echinococcosis or hydatidosis, which is one of the most widespread zoonotic infections of veterinary and medical importance. Numerous studies have shown that E. granulosus exists as a complex of strains differing in a wide variety of criteria. Ten distinct genotypes (G1-G10) have been identified with potential impact on the pathology, epidemiology and the effect of the measures implemented for the control of hydatidosis. Our main objective was to carry out a preliminary analysis of the genotypes of E. granulosus circulating in the central inland region of Portugal. Parasite samples (hydatid cysts, n=27) were isolated from the liver and lung of sheep and cattle. The DNA extracted from protoscoleces isolated from the fertile cysts served a template for the PCR amplification of part of the mitochondrial cytochrome c oxidase subunit 1 (cox1), ATP synthase F0 subunit 6 (atp6) as well as the large (rrnL/16S) and small (rrnS/12S) ribosomal RNA genes. Similarity searches with homologous sequences in the databanks indicated very high similarity with references assigned to the G1, G3 and/or G1-G3 complex of Echinococcus strains. Phylogenetic analysis (Bayesian approach) supported these observations, and confirmed the assignment of all the analyzed sequences to the G1-G3 genetic cluster. | por |
| dc.identifier.citation | BEATO, Sílvia Filipa Alves [et al.] (2010) - Apparent dominance of the G1-G3 genetic cluster of echinococcus granulosus strains in the central inland region of Portugal. Parasitology Internacional. ISSN 1383-5769. Vol. 59, nº 4. p. 638-642 | por |
| dc.identifier.issn | 1383-5769 | |
| dc.identifier.uri | http://hdl.handle.net/10400.11/1592 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S1383576910001352 | por |
| dc.subject | Echinococcus granulosus | por |
| dc.subject | Hydatic cyst | por |
| dc.subject | G1-G3 genotypes | por |
| dc.subject | Portugal | por |
| dc.subject | Mitochondrial DNA | por |
| dc.title | Apparent dominance of the G1-G3 genetic cluster of echinococcus granulosus strains in the central inland region of Portugal | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 642 | por |
| oaire.citation.startPage | 638 | por |
| oaire.citation.title | Parasitology International | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |