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Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugal

2014Journal article Restricted access
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dc.contributor.authorMatos, Ana Cristina
dc.contributor.authorFigueira, Luís
dc.contributor.authorMartins, Maria Helena
dc.contributor.authorLoureiro, Filipa
dc.contributor.authorPinto, Maria de Lurdes
dc.contributor.authorMatos, Manuela
dc.contributor.authorCoelho, Ana Cláudia
dc.date.accessioned2016-03-04T12:30:15Z
dc.date.available2016-03-04T12:30:15Z
dc.date.issued2014
dc.description.abstractA survey to determine the occurrence of Mycobacterium avium subsp. paratuberculosis (MAP) in wild carnivores in Portugal was conducted by testing samples from road-killed animals between 2009 and 2012. Postmortem examinations were performed and tissues were collected from wild carnivores representing four families and six different species, with a total of 74 animals analyzed. Cultures were performed by using Löwenstein-Jensen and Middlebrook 7H11 solid media and acid-fast isolates were identified by polymerase chain reaction (PCR) and mycobactin dependency characteristics. Tissues were also screened for MAP by directly extracting DNA and testing for the MAP-specific sequences. The occurrence of infected animals (an animal had at least one tissue that was positive for culture or direct PCR) was 27.0% (n = 20). MAP was isolated from culture of 25 tissue samples (3.8%) and was detected by direct PCR in 40 (6.0%) samples. Infection was recorded in 5/6 studied species: 7/49 (14.3%) red foxes (Vulpes vulpes), 3/3 (100%) beech martens (Martes foina), 2/4 (50.0%) Eurasian otters (Lutra lutra), 7/15 (46.7%) Egyptian mongooses (Herpestes ichneumon), and 1/1 (100%) European badger (Meles meles). These species represent three different taxonomic families: Canidae (14.3% were positive), Mustelidae (75.0% were positive), and Herpestidae (46.7% were positive). The results of this study confirm the presence of MAP infection in wild carnivores in Portugal.pt_PT
dc.identifier.citationMATOS, A.C [et al.] (2014). Survey of Mycobacterium avium subspecies paratuberculosis in road killed wild carnivores in Portugal. Journal of Zoo and Wildlife Medicine. 45 (4): 775-781. DOI 10.1638/2014-0010.1.pt_PT
dc.identifier.doi10.1638/2014-0010.1pt_PT
dc.identifier.issn1937-2825
dc.identifier.urihttp://hdl.handle.net/10400.11/3331
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttp://zoowildlifejournal.com/doi/abs/10.1638/2014-0010.1pt_PT
dc.subjectCulturept_PT
dc.subjectEpidemiologypt_PT
dc.subjectMycobacterium avium subsp. paratuberculosispt_PT
dc.subjectPCRpt_PT
dc.subjectWild carnivorespt_PT
dc.titleSurvey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceUSApt_PT
oaire.citation.endPage781pt_PT
oaire.citation.startPage775pt_PT
oaire.citation.titleJournal of Zoo and Wildlife Medicinept_PT
oaire.citation.volume45pt_PT
person.familyNameMatos
person.familyNameFigueira
person.familyNameFernandes Loureiro
person.familyNameRibeiro Pinto
person.familyNamede Matos
person.familyNameCoelho
person.givenNameAna
person.givenNameLuís Manuel Faria
person.givenNameFilipa Raquel
person.givenNameMaria de Lurdes
person.givenNameMaria Manuela Outeiro
person.givenNameAna
person.identifier84738
person.identifier.ciencia-id2711-E07A-FE45
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person.identifier.ciencia-id4F1E-ACFF-86CB
person.identifier.ciencia-id1E10-BF40-29C5
person.identifier.orcid0000-0001-9709-862X
person.identifier.orcid0000-0002-8454-2880
person.identifier.orcid0000-0002-5370-5333
person.identifier.orcid0000-0002-5928-6483
person.identifier.orcid0000-0002-9584-6636
person.identifier.orcid0000-0002-7196-4179
person.identifier.ridK-6664-2013
person.identifier.ridK-4740-2013
person.identifier.scopus-author-id55626078200
person.identifier.scopus-author-id34970835100
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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