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Properties of whey protein-based films containing organic acids and nisin to control listeria monocytogenes

dc.contributor.authorPintado, Cristina Santos
dc.contributor.authorFerreira, Maria A.S.S.
dc.contributor.authorSousa, Isabel
dc.date.accessioned2022-02-08T12:39:20Z
dc.date.available2022-02-08T12:39:20Z
dc.date.issued2009
dc.date.updated2022-02-07T01:37:45Z
dc.description.abstractWhey protein isolate and glycerol were mixed to form a matrix to incorporate antimicrobial agents and produce edible films with antimicrobial activity against Listeria monocytogenes strains isolated from cheeses. Various organic acids were used to decrease pH down to approximately 3. In a preliminary assay without nisin, the effect of each organic acid was evaluated with respect to the rheological properties of the film solutions and the inhibitory and mechanical properties of the films. Lactic, malic, and citric acids (3%, wt/vol), which were used in a subsequent study of their combined inhibitory effect with nisin (50 IU/ml), had significantly higher antilisterial activity (P , 0.05) compared with the control (2 N HCl, 3% [wt/vol], with nisin). The largest mean zone of inhibition was 4.00 6 0.92 mm for malic acid with nisin. Under small-amplitude oscillatory stress, the proteinglycerol-acid film solutions exhibited a predominantly viscous behavior or a weak gel behavior, with the storage modulus (G9) slightly higher than the loss modulus (G0). The malic acid–based solution was the only one whose viscosity was not influenced by the addition of nisin. The addition of nisin resulted in a nonsignificant (P . 0.05) increase in the percentage of elongation at break. Results from tensile and puncture stress were variable, but in general no significant differences were found after the incorporation of nisin. The overall results support the use of malic acid with nisin to produce effective antimicrobial films to control L. monocytogenes growth.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.eid2-s2.0-70349413160
dc.identifier.slugcv-prod-2597190
dc.identifier.urihttp://hdl.handle.net/10400.11/7883
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectWhey protein–based filmspt_PT
dc.subjectListeria monocytogenespt_PT
dc.titleProperties of whey protein-based films containing organic acids and nisin to control listeria monocytogenespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1896pt_PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1891pt_PT
oaire.citation.titleJournal of Food Protectionpt_PT
oaire.citation.volume72pt_PT
person.familyNameSantos Pintado
person.givenNameCristina Maria Baptista
person.identifier.ciencia-id7F1F-E7E1-5BC6
person.identifier.orcid0000-0001-5433-4258
person.identifier.scopus-author-id6602633775
rcaap.cv.cienciaid7F1F-E7E1-5BC6 | Cristina Maria Baptista Santos Pintado
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione5822f39-6e57-4619-b731-b51b3026cdfe
relation.isAuthorOfPublication.latestForDiscoverye5822f39-6e57-4619-b731-b51b3026cdfe

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