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Detection of Mycobacterium avium subsp. paratuberculosis in kidney samples of red deer (Cervus elaphus) in Portugal: Evaluation of different methods

2017Journal article Restricted access
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dc.contributor.authorMatos, Ana Cristina
dc.contributor.authorFigueira, Luís
dc.contributor.authorMartins, Maria Helena
dc.contributor.authorMatos, Manuela
dc.contributor.authorÁlvares, Sofia
dc.contributor.authorMendes, Andreia
dc.contributor.authorPinto, Maria de Lurdes
dc.contributor.authorCoelho, Ana Cláudia.
dc.date.accessioned2019-11-22T23:57:42Z
dc.date.available2019-11-22T23:57:42Z
dc.date.issued2017
dc.description.abstractParatuberculosis or Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (Map), is a chronic granulomatous enteritis affecting both domestic and wild ruminants. The present work is part of a wider set of studies designed to assess the prevalence of paratuberculosis in free ranging red deer (Cervus elaphus). With that purpose, 877 free-ranging red deer legally hunted in the Centre-eastern Portugal were submitted to necropsy and sampled for molecular methods, microbiology and histopathology. Thirty-seven (4.2%) kidneys revealed acid-fast bacilli when screened with the Ziehl-Neelsen technique. Map was detected by IS900 polymerase chain reaction (PCR) in thirty (81.1%) of the Ziehl-Neelsen positive kidneys. Subsequent PCR and/or culture from the different organs of the 37 examined animals allowed us to detect 86.4% (32 animals) infected red deer. Our results suggest that renal involvement in Map infected deer may be underdiagnosed and thus the routine examination of this organ and its inclusion in PCR techniques designed for Map detection could substantially improve the diagnostic of paratuberculosis in red deer.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMATOS, A.C., Luis FIGUEIRA, Maria H. MARTINS, Manuela MATOS, Sofia ÁLVARES, Andreia MENDES, Maria L. PINTO, Ana C. COELHO, Detection of Mycobacterium avium subsp. paratuberculosis in kidney samples of red deer (Cervus elaphus) in Portugal: Evaluation of different methods, Journal of Veterinary Medical Science, 2017, Volume 79, Issue 3, Pages 692-698, Released March 28, 2017, [Advance publication] Released January 30, 2017, Online ISSN 1347-7439, Print ISSN 0916-7250, https://doi.org/10.1292/jvms.16-0153, https://www.jstage.jst.go.jp/article/jvms/79/3/79_16-0153/_article/-char/en,pt_PT
dc.identifier.doihttps://doi.org/10.1292/jvms.16-0153pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.11/6771
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherJapanese Society of Veterinary Sciencept_PT
dc.relation.publisherversionhttps://www.jstage.jst.go.jp/article/jvms/79/3/79_16-0153/_articlept_PT
dc.subjectHistopathologypt_PT
dc.subjectKidneypt_PT
dc.subjectMycobacterium avium subsp. paratuberculosispt_PT
dc.subjectPCRpt_PT
dc.subjectRed deerpt_PT
dc.titleDetection of Mycobacterium avium subsp. paratuberculosis in kidney samples of red deer (Cervus elaphus) in Portugal: Evaluation of different methodspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage698pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage692pt_PT
oaire.citation.titleJournal of Veterinary Medical Sciencept_PT
oaire.citation.volume79pt_PT
person.familyNameMatos
person.familyNameFigueira
person.familyNamede Matos
person.familyNameRibeiro Pinto
person.familyNameCoelho
person.givenNameAna
person.givenNameLuís Manuel Faria
person.givenNameMaria Manuela Outeiro
person.givenNameMaria de Lurdes
person.givenNameAna
person.identifier84738
person.identifier.ciencia-id2711-E07A-FE45
person.identifier.ciencia-id941B-C381-8158
person.identifier.ciencia-id4F1E-ACFF-86CB
person.identifier.ciencia-idE81F-5A9E-5C8D
person.identifier.ciencia-id1E10-BF40-29C5
person.identifier.orcid0000-0001-9709-862X
person.identifier.orcid0000-0002-8454-2880
person.identifier.orcid0000-0002-9584-6636
person.identifier.orcid0000-0002-5928-6483
person.identifier.orcid0000-0002-7196-4179
person.identifier.ridK-6664-2013
person.identifier.ridK-4740-2013
person.identifier.scopus-author-id55626078200
person.identifier.scopus-author-id34970835100
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication74ef6ec4-da08-461e-a861-a4f5beb24858
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