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Natural extracts from Pterospartum tridentatum at different vegetative stages: extraction yiels, phenolic content and antioxidant activity
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Advisor(s)
Abstract(s)
The aerial parts of Pterospartum tridentatum, a wild growing species in Portugal used in
traditional medicine and gastronomy, were harvested at different stages (vegetative phase, flowering
phase and beginning of dormancy) in two locations in Portugal (Malcata and Gardunha mountains),
and the respective aqueous extracts have been studied. The influence of the seasonal variation in the
extraction yield, total phenolic content and antioxidant activity was evaluated. The extraction was
carried out in boiling water in consecutive steps. After each step, the aqueous extract was separated
and fresh water was added maintaining the same plant material. The procedure was repeated seven
times, within an overall time period of 180 minutes.
Higher extraction yields were achieved with plant stems collected at the vegetative phases, either from
Malcata or Gardunha regions. The total phenolic content of the extracts from Malcata plants ranged
from 273 mg to 400 mg gallic acid equivalent/g dry matter, which was quite similar to that determined
for extracts from Gardunha (245 to 394 mg gallic acid equivalent/g dry matter). The antioxidant activity
was determined by the radical scavenging activity method using 2,2-diphenyl-1-picrylhydrazyl radical
(DPPH). The greatest radical scavenging activity was observed in the flowers extracts, even though all
extracts produced presented a good antioxidant activity. Furthermore, the antioxidant activity was not
affected by the exposure of the plant material at 100ºC for long periods of time (180 min).
The results show that Pterospartum tridentatum has a great potential to be used as a new source of
natural antioxidants for the food industry.
Description
Keywords
Pterospartum tridentatum Aqueous extracts Extraction yield Antioxidant activity Phenolic content
Citation
COELHO, M.T. [et al.] (2012) - Natural extracts from Pterospartum tridentatum at different vegetative stages: extraction yiels, phenolic content and antioxidant activity. In Central European Congress on Food, 6th, Novi Sad, 23- 26 May - Proceedings. [S.l. : s.n.]. P. 24-28.