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Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment
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Orientador(es)
Resumo(s)
We compared the genetic variation of Pinus pinaster populations using amplified fragment
length polymorphism (AFLP) and chloroplast simple-sequence repeat (cpSSR) loci. Populations’ levels of diversity within groups were found to be similar with AFLPs, but not with cpSSRs. The high interlocus variance associated with the AFLP loci could account for the lack of differences in the former. Although AFLPs revealed much lower genetic diversity
than cpSSRs, the levels of among-population differentiation found with the two types of
marker were similar, provided that loci showing fewer than four null-homozygotes, in any
population, were pruned from the AFLP data. Moreover, the French and Portuguese populations were clearly differentiated from each other, with both markers. The Mantel test
showed that the genetic distance matrix calculated using the AFLP data was correlated with
the matrix derived from the cpSSRs. Because of the concordance found between markers
we conclude that gene flow was indeed the predominant force shaping nuclear and chloroplastic genetic variation of the populations within regions, at the geographical scale studied.
Descrição
This document is available at http://www.wiley.com/WileyCDA/
Palavras-chave
AFLP CPSSR Chloroplast microsatellites Gene diversity Population differentiation Pinus pinaster
Contexto Educativo
Citação
RIBEIRO, M.M. [et al.] (2002) - Comparison of genetic diversity estimates within and among populations of maritime pine using chloroplast simple-sequence repeat and amplified fragment, Molecular Ecology. ISSN 0962-1083. 11, p. 869-877.