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Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease

2021Journal article Open access
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dc.contributor.authorMendes, Rafael J.
dc.contributor.authorSario, Sara
dc.contributor.authorLuz, J.P.
dc.contributor.authorFagundes, Natália
dc.contributor.authorTeixeira, Cátia
dc.contributor.authorGomes, Paula
dc.contributor.authorTavares, Fernando
dc.contributor.authorSantos, Conceição
dc.date.accessioned2023-03-28T15:13:33Z
dc.date.available2023-03-28T15:13:33Z
dc.date.issued2021
dc.description.abstractFire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees. This disease is caused by Erwinia amylovora, a quarantine phytopathogenic bacterium, which, although highly distributed worldwide, still lacks efficient control measures. The green revolution paradigm demands sustainable agriculture practices, for which antimicrobial peptides (AMPs) have recently caught much attention. The goal of this work was to disclose the bioactivity of three peptides mixtures (BP100:RW-BP100, BP100:CA-M, and RWBP100: CA-M), against three strains of E. amylovora representing distinct genotypes and virulence (LMG 2024, Ea 630 and Ea 680). The three AMPs’ mixtures were assayed at eight different equimolar concentrations ranging from 0.25 to 6 M (1:1). Results showed MIC and MBC values between 2.5 and 4 M for every AMP mixture and strain. Regarding cell viability, flow cytometry and alamarBlue reduction, showed high reduction (>25%) of viable cells after 30 min of AMP exposure, depending on the peptide mixture and strain assayed. Hypersensitive response in tobacco plants showed that the most efficient AMPs mixtures and concentrations caused low to no reaction of the plant. Altogether, the AMPs mixtures studied are better treatment solutions to control fire blight disease than the same AMPs applied individually.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMENDES, R.J. [et al.] (2021) - Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease. Plants. Vol.10:12, 2637. DOI http://hdl.handle.net/10400.11/8448
dc.identifier.doi10.3390/plants10122637pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.11/8448
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationPearFire: Functional omics of Erwinia-PearRocha pathosystem to characterize virulent strains and resistant pear genotypes
dc.relationDeciphering Drosophila suzukii-cherry interactions and development of new strategies for pest control
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationPEPALHEIRA - Whey proteins and derived antimicrobial PEPtide-based active edible coatings for ALHEIRA industry
dc.relationTeaming to Upgrade to Excellence in Environmental Biology, Ecosystem Research and AgroBiodiversity
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAlamarBlue reductionpt_PT
dc.subjectAMPspt_PT
dc.subjectBP100pt_PT
dc.subjectCA-Mpt_PT
dc.subjectErwinia amylovorapt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectGreen revolutionpt_PT
dc.subjectHypersensitive responsept_PT
dc.subjectRW-BP100pt_PT
dc.subjectSustainable controlpt_PT
dc.titleEvaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight diseasept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePearFire: Functional omics of Erwinia-PearRocha pathosystem to characterize virulent strains and resistant pear genotypes
oaire.awardTitleDeciphering Drosophila suzukii-cherry interactions and development of new strategies for pest control
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitlePEPALHEIRA - Whey proteins and derived antimicrobial PEPtide-based active edible coatings for ALHEIRA industry
oaire.awardTitleTeaming to Upgrade to Excellence in Environmental Biology, Ecosystem Research and AgroBiodiversity
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F133519%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F138186%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBAA-AGR%2F31798%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/857251/EU
oaire.citation.issue12pt_PT
oaire.citation.startPage2637pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStreamPOR_NORTE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamH2020
person.familyNameMendes
person.familyNamePais Sario
person.familyNameLuz
person.familyNameTassi Campos Procópio Fagundes
person.familyNameTeixeira
person.familyNameGomes
person.familyNameTavares
person.familyNameDos Santos
person.givenNameRafael
person.givenNameSara Judite
person.givenNameJoão Pedro
person.givenNameNatália
person.givenNameCátia
person.givenNamePaula
person.givenNameFernando
person.givenNameConceição Domingos José
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person.identifier.scopus-author-id7005803189
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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