Chemical and physical parameters of Portuguese honey : classification of Citrus, Erica, Lavandula and Eucalyptus honeys by multivariate analysis and FTIR-ATR spectroscopy

Anjos, Ofélia; Correia, Liliana; Gouveia, Cecília; Vitorino, Conceição; Rodrigues, José Carlos; Peres, Maria de Fátima

http://hdl.handle.net/10400.11/285

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Authors

Rodrigues, José Carlos

Peres, Maria de Fátima

Abstract(s)

In this study eight samples of monofloral honey from Portugal, selected from four different botanical origins: Eucalyptus spp., Erica spp., Lavandula spp. And Citrus spp. were studied. For each monofloral honey two samples from different producers were analysed. Several analyses were carried out: moisture content, water activity (aw). Colour, ash, pH, free acidity, electrical conductivity and total phenol content. All the data were statistically tested using variance analysis and principal component analysis (PCA) with the aim of classifying the honeys and identifying the most significant parameters in the classification. Measurements with FTIR-ATR spectroscopy were performed on order to correlate the previously chemical parameter and the obtained spectra. The one-way analysis of variance, considering the factor botanical origin shows that, in general, this factor is highly significant. In particular it can be stated (p<0.001) that is occurs in almost all variables, that explained between 81% and 98% of the total variance. From the statistical study by PCA the variables analyzed, two factors explain 86.3% of the total variance between honey types. We can find a good correlation between ash, pH, free acidity, electrical conductivity, colour and total phenol content parameter that was explained by the factor 1 (66.6%). The moisture content and aw are explained by the second factor (19.8%) and are not correlated with the previously factor. In fact the water activity is a variable rarely described as discriminant for the different honey types. PCA analysis shows that a good separation between three groups: 1 – Eucalyptus honey; 2 – Erica honey; 3- Lavandula and Citrus honeys. Usually the Lavandula species and Citrus species growth in similar habitats, consequently present a great percentage of both kind of pollens. In fact with the Scheffé test only the ash content and electrical conductivity are significantly different from these monofloral honeys.

Description

Trabalho apresentado na 3rd Scientific Conference of Institute of Tropics and Subtropics, que decorreu em Novembro de 2009 em Praga.

Keywords

Honey Botanical origin Physico-chemical analysis FTIR-ATR spectra

URI

http://hdl.handle.net/10400.11/285

Citation

ANJOS, O. [et al.] (2009) - Chemical and physical parameters of Portuguese honey : classification of Citrus, Erica, Lavandula and Eucalyptus honeys by multivariate analysis and FTIR-ATR spectroscopy. In Scientific Conference of Institute of Tropics and Subtropics, 3, Prague, 19 November - Sustainable use of natural resources in tropics and subtropics : book of abstracts. Prague : CULS. Institute of Tropics and Subtropics. ISBN 978-80-213-2009-2. p. 31-32.