Percorrer por autor "Breitenfeld, Luísa"
A mostrar 1 - 3 de 3
Resultados por página
Opções de ordenação
- Determinação de potenciais biomarcadores de infertilidade feminina : estudo pilotoPublication . Brinca, Ana Teresa; Anjos, O.; Alves, Maria Manuel; Sousa, Ângela; Oliani, António Hélio; Breitenfeld, Luísa; Passarinha, Luís A.; Ramalhinho, Ana Cristina; Gallardo, EugeniaDevido à sua elevada incidência, a infertilidade tornou-se uma questão de saúde pública proeminente, representando um desafio significativo para a medicina reprodutiva moderna.
- Humulus lupulus aqueous extract and hydrolate as a potential ingredient for cosmetics : chemical characterization and in vitro antimicrobial, cytotoxicity, antioxidant, and anti-inflammatory assessmentPublication . Valente, João Vasco; Oliveira, Rita Palmeira de; Guiomar, Liliana; Vaz, Cátia; Rolo, Joana; Gaspar, Carlos; Oliveira, Ana Sofia; Caramelo, Débora; Breitenfeld, Luísa; Gonçalves, José Carlos; Delgado, F.M.G.; Oliveira, José Martinez; Oliveira, Ana Palmeira deHumulus lupulus extracts have in their composition different molecules, such aspolyphenols, α-acids, β -acids, and hydrocarbons, which contribute to the plant’s medicinal properties. These molecules are associated with antimicrobial, antioxidant, and anti - inflammatory activities. OBJECTIVE This work focuses on the evaluation of H. lupulus biological activities, with the aim of evaluating its potential for inclusion in cosmetic formulations. METHODS Two distinct aqueous extracts and two hydrolates obtained via hydrodistillation were evaluated. These include the flower parts (FE, FH) and the mix of aboveground parts (ME, MH). The chemical profiles for both aqueous extracts and hydrolates were identified by high performance liquid chromatography (HPLC chromatography -mass spectrometry (GC -MS ). Antimicrobial, antioxidant, cytotoxicity , and anti-inflammatory activity were tested in vitro using standard methods. RESULTS Rutin was the major compound found in FE (40.041 µg mg -1 of extract) and ME (2.909 µg mg -1 of extract), while humulenol II and was the most abundant compound in hydrolates (FH:20.83%; MH: 46.80%). Furthermore, FE was able to inhibit the growth of Staphylococcus aureus and Staphylococcus epidermis with MIC values of 50% and 25% (v/v), respectively. FH showed the same effect Staphylococcus aureus (50% v/v). FH evidenced poor antioxidant potential in DPPH scavenging test, it demonstrated significant antioxidant and anti-inflammatory effects by reducing (***p<0.001) intracellular reactive oxygen species (ROS), NO (nitric oxide) levels (***p<0.001) and cyclooxygenase -2 (COX-2) protein expression (***p<0.001) in lipopolysaccharide (LPS) -stimulated macrophages. Nevertheless, it is important to note that FH exhibited cytotoxicity at high concentrations in 3T3 fibroblasts and RAW macrophages. CONCLUSION The studied H. lupulus aqueous extracts and hydrolates revealed that FH stands out as the most promising bioactive source for cosmetic formulations. However, future research addressing antimicrobial activity is necessary to confirm its potential incorporation into dermatological and cosmetic formulations.
- Volatilomics as an emerging strategy to determine potential biomarkers of female infertility: a pilot studyPublication . Brinca, Ana Teresa; Anjos, O.; Alves, Maria Manuel; Sousa, Ângela; Oliani, António Hélio; Breitenfeld, Luísa; Passarinha, Luís A.; Ramalhinho, Ana Cristina; Gallardo, EugeniaDue to its high prevalence, infertility has become a prominent public health issue, posing a significant challenge to modern reproductive medicine. Some clinical conditions that lead to female infertility include polycystic ovary syndrome (PCOS), endometriosis, and premature ovarian failure (POF). Follicular fluid (FF) is the biological matrix that has the most contact with the oocyte and can, therefore, be used as a predictor of its quality. Volatilomics has emerged as a non-invasive, straightforward, affordable, and simple method for characterizing various diseases and determining the effectiveness of their current therapies. In order to find potential biomarkers of infertility, this study set out to determine the volatomic pattern of the follicular fluid from patients with PCOS, endometriosis, and POF. The chromatographic data integration was performed through solid-phase microextraction (SPME), followed by gas chromatography–mass spectrometry (GC-MS). The findings pointed to specific metabolite patterns as potential biomarkers for the studied diseases. These open the door for further research into the relevant metabolomic pathways to enhance infertility knowledge and diagnostic tools. An extended investigation may, however, produce a new mechanistic understanding of the pathophysiology of the diseases.