Browsing by Author "Brinca, Ana Teresa"
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- Determinação de potenciais biomarcadores de infertilidade feminina : estudo pilotoPublication . Brinca, Ana Teresa; Anjos, O.; Alves, Maria Manuel; Sousa, Ângela; Oliani, António Hélio; Breitenfeld, Luísa; Passarinha, Luís A.; Ramalhinho, Ana Cristina; Gallardo, EugeniaDevido à sua elevada incidência, a infertilidade tornou-se uma questão de saúde pública proeminente, representando um desafio significativo para a medicina reprodutiva moderna.
- Miniaturized solid phase extraction techniques applied to natural productsPublication . Rosendo, Luana M.; Brinca, Ana Teresa; Pires, Bruno; Catarro, Gonçalo; Rosado, Tiago; Guiné, Raquel P.F.; Araújo, André; Anjos, O.; Gallardo, EugeniaNatural products are increasingly becoming part of our daily lives through their use in industry, food, as therapeutic agents, etc. To evaluate their possible applications, it is essential to characterize them chemically to explore their potential. Different techniques may be used to characterize natural products, including microextraction techniques. These techniques have been gaining popularity due to the advantages associated with their low use of organic solvents and the small amount of sample used relative to more classical sample preparation techniques. Their application in the extraction of compounds from natural products is still scarce. This manuscript intends to review the most used solid-based miniaturized sample preparation techniques applied to determining compounds in natural products. The main applications of these methodologies will be discussed, with a particular focus on natural product analysis, as well as their advantages and disadvantages over traditionally used sample preparation techniques.
- Miniaturized solid phase extraction techniques applied to natural productsPublication . Rosendo, Luana M.; Brinca, Ana Teresa; Pires, Bruno; Catarro, Gonçalo; Rosado, Tiago; Martinho, J.P.; Guiné, Raquel P.F.; Araújo, André; Anjos, O.; Galhardo, EugeniaNatural products are increasingly becoming part of our daily lives through their use in industry, food, as therapeutic agents, etc. Different techniques may be used to characterize natural products, including microextraction techniques. This work intends to review the most used solid-based miniaturized sample preparation techniques applied to determining compounds in natural products. Advantages and drawbacks are also presented. A
- Volatilomics as an emerging strategy to determine potential biomarkers of female infertility: a pilot studyPublication . Brinca, Ana Teresa; Anjos, O.; Alves, Maria Manuel; Sousa, Ângela; Oliani, António Hélio; Breitenfeld, Luísa; Passarinha, Luís A.; Ramalhinho, Ana Cristina; Gallardo, EugeniaDue to its high prevalence, infertility has become a prominent public health issue, posing a significant challenge to modern reproductive medicine. Some clinical conditions that lead to female infertility include polycystic ovary syndrome (PCOS), endometriosis, and premature ovarian failure (POF). Follicular fluid (FF) is the biological matrix that has the most contact with the oocyte and can, therefore, be used as a predictor of its quality. Volatilomics has emerged as a non-invasive, straightforward, affordable, and simple method for characterizing various diseases and determining the effectiveness of their current therapies. In order to find potential biomarkers of infertility, this study set out to determine the volatomic pattern of the follicular fluid from patients with PCOS, endometriosis, and POF. The chromatographic data integration was performed through solid-phase microextraction (SPME), followed by gas chromatography–mass spectrometry (GC-MS). The findings pointed to specific metabolite patterns as potential biomarkers for the studied diseases. These open the door for further research into the relevant metabolomic pathways to enhance infertility knowledge and diagnostic tools. An extended investigation may, however, produce a new mechanistic understanding of the pathophysiology of the diseases.