Percorrer por autor "Oliveira, Rita Palmeira de"
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- Bioactivity of Matricaria chamomilla, Echinacea purpurea, Thymbra capitata and Ocimum basilicum hydrolates and essential oils in view of their application in the skinPublication . Valente, João Vasco; Gomes, Carolina Proença; Oliveira, Ana Sofia; Rolo, Joana; Gaspar, Carlos; Caramelo, Débora; Gonçalves, José Carlos; Delgado, F.M.G.; Breitenfeld, Luisa; Duarte, Ana Paula; Oliveira, Rita Palmeira de; Oliveira, José Martinez de; Oliveira, Ana Palmeira deThe numerous health benefits associated with the use of plants in traditional medicine can be linked to secondary metabolites in the products obtained through the hydrodistillation process, such as hydrolates and essential oils (EOs). This study aimed to assess the chemical profile and the antioxidant, antimicrobial, cytotoxic, and anti-inflammatory activities of two hydrodistillation products obtained from four plants belonging to two distinct families: chamomile (Matricaria chamomilla), purple coneflower (Echinacea purpurea), conehead thyme (Thymbra capitata), and basil (Ocimum basilicum). The EOs were found to be more effective than hydrolates in inhibiting microorganisms’ growth, registering MIC values equal to or higher than 50% (v/v), except for T. capitata. Only T. capitata demonstrated the ability to reduce NO levels with both hydrolate and EO. Moreover, it inhibited the cell growth of RAW 264.7, 3T3, and HaCaT lines at the tested concentrations. In contrast, O. basilicum EO did not affect the cell proliferation of the tested cell lines in concentrations below 0.063% (v/v) and showed a significant reduction in the macrophage NO production at all concentrations. Thus, EOs showed a superior biological activity than hydrolates. In particular, O. basilicum EO was found to be a promising candidate for therapeutic applications on the skin.
- Humulus lupulus aqueous extract and hydrolate as a potential ingredient for cosmetics : chemical characterization and in vitro antimicrobial, cytotoxicity, antioxidant, and anti-inflammatory assessmentPublication . Valente, João Vasco; Oliveira, Rita Palmeira de; Guiomar, Liliana; Vaz, Cátia; Rolo, Joana; Gaspar, Carlos; Oliveira, Ana Sofia; Caramelo, Débora; Breitenfeld, Luísa; Gonçalves, José Carlos; Delgado, F.M.G.; Oliveira, José Martinez; Oliveira, Ana Palmeira deHumulus lupulus extracts have in their composition different molecules, such aspolyphenols, α-acids, β -acids, and hydrocarbons, which contribute to the plant’s medicinal properties. These molecules are associated with antimicrobial, antioxidant, and anti - inflammatory activities. OBJECTIVE This work focuses on the evaluation of H. lupulus biological activities, with the aim of evaluating its potential for inclusion in cosmetic formulations. METHODS Two distinct aqueous extracts and two hydrolates obtained via hydrodistillation were evaluated. These include the flower parts (FE, FH) and the mix of aboveground parts (ME, MH). The chemical profiles for both aqueous extracts and hydrolates were identified by high performance liquid chromatography (HPLC chromatography -mass spectrometry (GC -MS ). Antimicrobial, antioxidant, cytotoxicity , and anti-inflammatory activity were tested in vitro using standard methods. RESULTS Rutin was the major compound found in FE (40.041 µg mg -1 of extract) and ME (2.909 µg mg -1 of extract), while humulenol II and was the most abundant compound in hydrolates (FH:20.83%; MH: 46.80%). Furthermore, FE was able to inhibit the growth of Staphylococcus aureus and Staphylococcus epidermis with MIC values of 50% and 25% (v/v), respectively. FH showed the same effect Staphylococcus aureus (50% v/v). FH evidenced poor antioxidant potential in DPPH scavenging test, it demonstrated significant antioxidant and anti-inflammatory effects by reducing (***p<0.001) intracellular reactive oxygen species (ROS), NO (nitric oxide) levels (***p<0.001) and cyclooxygenase -2 (COX-2) protein expression (***p<0.001) in lipopolysaccharide (LPS) -stimulated macrophages. Nevertheless, it is important to note that FH exhibited cytotoxicity at high concentrations in 3T3 fibroblasts and RAW macrophages. CONCLUSION The studied H. lupulus aqueous extracts and hydrolates revealed that FH stands out as the most promising bioactive source for cosmetic formulations. However, future research addressing antimicrobial activity is necessary to confirm its potential incorporation into dermatological and cosmetic formulations.