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In vitro evaluation of five antimicrobial peptides against the plant pathogen Erwinia amylovora

2021Journal article Open access
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dc.contributor.authorMendes, Rafael
dc.contributor.authorRegalado, Laura
dc.contributor.authorLuz, J.P.
dc.contributor.authorTassi, Natália
dc.contributor.authorTeixeira, Cátia
dc.contributor.authorGomes, Paula
dc.contributor.authorTavares, Fernando
dc.contributor.authorSantos, Conceição
dc.date.accessioned2021-04-23T11:31:25Z
dc.date.available2021-04-23T11:31:25Z
dc.date.issued2021
dc.description.abstractFire blight is a major pome fruit trees disease that is caused by the quarantine phytopathogenic Erwinia amylovora, leading to major losses, namely, in pear and apple productions. Nevertheless, no effective sustainable control treatments and measures have yet been disclosed. In that regard, antimicrobial peptides (AMPs) have been proposed as an alternative biomolecule against pathogens but some of those AMPs have yet to be tested against E. amylovora. In this study, the potential of five AMPs (RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) together with BP100, were assessed to control E. amylovora. Antibiograms, minimal inhibitory, and bactericidal concentrations (minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), growth and IC50 were determined and membrane permeabilization capacity was evaluated by flow cytometry analysis and colony-forming units (CFUs) plate counting. For the tested AMPs, the higher inhibitory and bactericidal capacity was observed for RW-BP100 and CA-M (5 and 5–8 M, respectively for both MIC and MBC), whilst for IC50 RW-BP100 presented higher efficiency (2.8 to 3.5 M). Growth curves for the first concentrations bellow MIC showed that these AMPs delayed E. amylovora growth. Flow cytometry disclosed faster membrane permeabilization for CA-M. These results highlight the potential of RW-BP100 and CA-M AMPs as sustainable control measures against E. amylovora.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMENDES, R.J [et al.] (2021) - In vitro evaluation of five antimicrobial peptides against the plant pathogen Erwinia amylovora. Biomolecules. 11:4, p. 554-571. DOI https://doi.org/10.3390/biom11040554pt_PT
dc.identifier.doi10.3390/biom11040554pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.11/7518
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationFEDER UIDB/50006/2020pt_PT
dc.relationPearFire: Functional omics of Erwinia-PearRocha pathosystem to characterize virulent strains and resistant pear genotypes
dc.relationCOMPETE 2020pt_PT
dc.relationPEPALHEIRA - Whey proteins and derived antimicrobial PEPtide-based active edible coatings for ALHEIRA industry
dc.relation.publisherversionhttps://www.mdpi.com/journal/biomoleculespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntimicrobial activitypt_PT
dc.subjectCA-Mpt_PT
dc.subjectDhvar-5pt_PT
dc.subjectD4E1pt_PT
dc.subjectFire blightpt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectMembrane permeabilizationpt_PT
dc.subjectRW-BP100pt_PT
dc.titleIn vitro evaluation of five antimicrobial peptides against the plant pathogen Erwinia amylovorapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePearFire: Functional omics of Erwinia-PearRocha pathosystem to characterize virulent strains and resistant pear genotypes
oaire.awardTitlePEPALHEIRA - Whey proteins and derived antimicrobial PEPtide-based active edible coatings for ALHEIRA industry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F133519%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBAA-AGR%2F31798%2F2017/PT
oaire.citation.endPage571pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage554pt_PT
oaire.citation.titleBiomoleculespt_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamPOR_NORTE
oaire.fundingStream9471 - RIDTI
person.familyNameMendes
person.familyNameRegalado
person.familyNameLuz
person.familyNameTeixeira
person.familyNameGomes
person.familyNameTavares
person.familyNameDos Santos
person.givenNameRafael
person.givenNameLaura
person.givenNameJoão Pedro
person.givenNameCátia
person.givenNamePaula
person.givenNameFernando
person.givenNameConceição Domingos José
person.identifier192227
person.identifier.ciencia-idA712-701F-0EEC
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person.identifier.ciencia-id8F18-9278-8A02
person.identifier.ciencia-id551B-A54A-BC60
person.identifier.ciencia-idA61A-A0F5-77F8
person.identifier.orcid0000-0001-5212-6172
person.identifier.orcid0000-0002-6278-9727
person.identifier.orcid0000-0002-3494-737X
person.identifier.orcid0000-0001-9506-3781
person.identifier.orcid0000-0002-6018-4724
person.identifier.orcid0000-0001-9913-1155
person.identifier.ridM-7245-2013
person.identifier.ridC-6698-2008
person.identifier.ridC-3375-2013
person.identifier.scopus-author-id57191629737
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person.identifier.scopus-author-id23981482200
person.identifier.scopus-author-id7102879822
person.identifier.scopus-author-id7005803189
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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