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Publication
In vitro evaluation of five antimicrobial peptides against the plant pathogen Erwinia amylovora
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| In Vitro Evaluation of Five Antimicrobial Peptides against the Plant Pathogen Erwinia amylovora | 1.52 MB | Adobe PDF |
Advisor(s)
Abstract(s)
Fire blight is a major pome fruit trees disease that is caused by the quarantine phytopathogenic
Erwinia amylovora, leading to major losses, namely, in pear and apple productions.
Nevertheless, no effective sustainable control treatments and measures have yet been disclosed.
In that regard, antimicrobial peptides (AMPs) have been proposed as an alternative biomolecule
against pathogens but some of those AMPs have yet to be tested against E. amylovora. In this study,
the potential of five AMPs (RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) together with BP100, were
assessed to control E. amylovora. Antibiograms, minimal inhibitory, and bactericidal concentrations
(minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), growth and
IC50 were determined and membrane permeabilization capacity was evaluated by flow cytometry
analysis and colony-forming units (CFUs) plate counting. For the tested AMPs, the higher inhibitory
and bactericidal capacity was observed for RW-BP100 and CA-M (5 and 5–8 M, respectively for
both MIC and MBC), whilst for IC50 RW-BP100 presented higher efficiency (2.8 to 3.5 M). Growth
curves for the first concentrations bellow MIC showed that these AMPs delayed E. amylovora growth.
Flow cytometry disclosed faster membrane permeabilization for CA-M. These results highlight the
potential of RW-BP100 and CA-M AMPs as sustainable control measures against E. amylovora.
Description
Keywords
Antimicrobial activity CA-M Dhvar-5 D4E1 Fire blight Flow cytometry Membrane permeabilization RW-BP100
Pedagogical Context
Citation
MENDES, R.J [et al.] (2021) - In vitro evaluation of five antimicrobial peptides against the plant pathogen Erwinia amylovora. Biomolecules. 11:4, p. 554-571. DOI https://doi.org/10.3390/biom11040554
Publisher
MDPI