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Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers
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Orientador(es)
Resumo(s)
Conventionally morphological descriptors are routinely used for establishing the
identity of varieties. This kind of descriptors has some disadvantages, namely most of them
are quantitative, controlled by several pairs of genes, and their expression is infl uenced by
environmental factors. Molecular markers have a potential to facilitate this procedure, increase
the reliability of decisions, and substantially save the time and space needed for experiments. In
this study we intended to identify 20 cultivars of fi eld pea (Pisum sativum L.), registered in the Community
Catalog of Varieties, by microsatellites molecular markers. After DNA extraction, seven different loci
were analyzed. PCR amplifi cations were conducted and the resulting fragments were separated on a 3.5%
MS-8 agarose gel in TBE buffer. The gels were analyzed for the presence/absence of bands and a table
with binary code was made. For each locus PIC value was calculated. The data were processed with the
statistical software NTSYS-pc, using the SIMQUAL module and Jaccard similarity coeffi cient, followed
by UPGMA cluster analysis. With the analysis of six polymorphic loci was possible to distinguish almost
all of cultivars. The most informative loci were AD61 and AB53. The UPGMA dendrogram showed two
main groups. The results showed a high potential and resolving power of SSR markers in distinct
assessment. SSR markers might also be useful in germplasm management and genetic diversity
studies. In the present research work, we have successfully employed high resolution agarose gel
electrophoresis for genotyping with microsatellite markers in pea.
Descrição
Palavras-chave
Cultivar identification DNA fingerprinting High resolution agarose gel
Contexto Educativo
Citação
REIS, C.M.G. ; DIOGO, M.G. (2012) - Identification of field pea cultivars (Pisum sativum L.) using micro-satellite molecular markers. In Genetics and Plant Physiology. ISSN ISSN 1314-6394. Vol. 2, n.º 1-2. p. 57-63.
Editora
Institute of Plant Physiology and Genetics – Bulgarian Academy of Sciences