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Sugar detection and quantification in honey bee and stingless bee honey — A review

datacite.subject.fosCiências Agrárias::Biotecnologia Agrária e Alimentar
dc.contributor.authorRessutte, Jéssica
dc.contributor.authorAnjos, O.
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorSpinosa, Wilma
dc.date.accessioned2026-05-28T11:52:16Z
dc.date.available2026-05-28T11:52:16Z
dc.date.issued2026
dc.descriptionTo the Plant Biotechnology Center of Beira Interior, CBP-BI and the Polytechnic Institute of Castelo Branco (IPCB) for welcoming me over the 5 months during which this study was conducted. We would like to acknowledge Natália Roque from the Polytechnic Institute of Castelo Branco for her valuable contribution to the development of Figure 1.
dc.description.abstractBee products are renowned for their beneficial properties and nutritional value. This association has been supported by scientific research that delves into their composition and biological activities. Given the increasing interest in natural products and the prevalence of fraudulent practices, understanding the sugar profiles of honeys is of paramount importance. In this review, we explore the sugars found in honey bee honey and stingless bee honey sourced from both floral nectar and honeydew. Additionally, our study provides a comprehensive review of direct and indirect methods of sugar analysis, highlighting the advantages and disadvantages of each method. Direct and indirect methods for sugar analysis include liquid and gas chromatography, vibrational spectroscopy, and nuclear magnetic resonance. These techniques form the foundation for the quality control of honey. High concentrations of trisaccharide and trehalulose have been identified as biomarkers of honeydew honey and stingless bee honey, respectively. In terms of liquid chromatography, mass spectrometry and pulsed amperometric detection are cited as the most suitable methods for the quantification of sugars in honey; however, the most widely used detector is the refractive index. Alternative methods such as vibrational spectroscopy and nuclear magnetic resonance, especially the latter, have also proved effective in quantifying sugar in honey.eng
dc.description.sponsorshipJéssica Ressutte thanks Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support (Grant numbers: 88882.448231/2019-01and 88881.624487/2021-01). Eugenia Gallardo is grateful to the Foundation for Science and Technology (FCT, Portugal) for their financial support RISE-Health UBI). Ofélia Anjos thanks the Foundation for Science and Technology (FCT,Portugal) for their financial support through national funds FCT/MCTES (PIDDAC) to CERNAS-IPCB, UID/681/2025.
dc.identifier.citationRESSUTTE, J. [et al.] (2026) - Sugar detection and quantification in honey bee and stingless bee honey — A review. Journal of Apicultural Research. 65:2, p. 262–288. DOI: 10.1080/00218839.2025.2581462
dc.identifier.doi10.1080/00218839.2025.2581462
dc.identifier.issn0021-8839
dc.identifier.issn2078-6913
dc.identifier.urihttp://hdl.handle.net/10400.11/10886
dc.language.isoeng
dc.peerreviewedyes
dc.publisherTaylor & Francis
dc.relation.ispartofJournal of Apicultural Research
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHoney
dc.subjectSugar analysis
dc.subjectSugars in honey
dc.subjectHoney biomarkers
dc.subjectQuality control
dc.titleSugar detection and quantification in honey bee and stingless bee honey — A revieweng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage288
oaire.citation.issue2
oaire.citation.startPage262
oaire.citation.titleJournal of Apicultural Research
oaire.citation.volume65
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameAnjos
person.givenNameOfélia
person.identifier.ciencia-idC21D-D8C7-3037
person.identifier.orcid0000-0003-0267-3252
person.identifier.ridG-2808-2012
person.identifier.scopus-author-id23395659700
relation.isAuthorOfPublicationdf9191ae-0bbb-4bb8-bbdc-0f79c7365876
relation.isAuthorOfPublication.latestForDiscoverydf9191ae-0bbb-4bb8-bbdc-0f79c7365876

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