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Estimating sequence diversity of prion protein gene (PRNP) in Portuguese populations of two cervid species: red deer and fallow deer

2023Journal article Open access
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dc.contributor.authorPereira, Jorge C.
dc.contributor.authorGonçalves-Anjo, Nuno
dc.contributor.authorOrge, Leonor
dc.contributor.authorPires, Maria dos Anjos
dc.contributor.authorRocha, Sara
dc.contributor.authorFigueira, Luís
dc.contributor.authorMatos, Ana Cristina
dc.contributor.authorSilva, João
dc.contributor.authorMendonça, Paula
dc.contributor.authorCarvalho, Paulo
dc.contributor.authorTavares, Paula
dc.contributor.authorLima, Carla
dc.contributor.authorAlves, Anabela
dc.contributor.authorEsteves, Alexandra
dc.contributor.authorPinto, Maria de Lurdes
dc.contributor.authorPires, Isabel
dc.contributor.authorGama, Adelina
dc.contributor.authorSargo, Roberto
dc.contributor.authorSilva, Filipe
dc.contributor.authorSeixas, Fernanda
dc.contributor.authorPinto, Madalena Vieira
dc.contributor.authorBastos, Estela
dc.date.accessioned2023-12-19T16:36:58Z
dc.date.available2023-12-19T16:36:58Z
dc.date.issued2023
dc.description.abstractAmong the transmissible spongiform encephalopathies (TSEs), chronic wasting disease (CWD) in cervids is now a rising concern in wildlife within Europe, after the detection of the first case in Norway in 2016, in a wild reindeer and until June 2022 a total of 34 cases were described in Norway, Sweden and Finland. The definite diagnosis is post-mortem, performed in target areas of the brain and lymph nodes. Samples are first screened using a rapid test and, if positive, confirmed by immunohistochemistry and Western immunoblotting. The study of the genetics of the prion protein gene, PRNP, has been proved to be a valuable tool for determining the relative susceptibility to TSEs. In the present study, the exon 3 of PRNP gene of 143 samples from red deer (Cervus elaphus) and fallow deer (Dama dama) of Portugal was analysed. Three single nucleotide polymorphisms (SNPs) were found in red deer – codon A136A, codon T98A, codon Q226E – and no sequence variation was detected in fallow deer. The low genetic diversity found in our samples is compatible with previous studies in Europe. The comparison with results from North America suggests that the free-ranging deer from our study may present susceptibility to CWD, although lack of experimental data and the necessity of continuous survey are necessary to evaluate these populations.pt_PT
dc.description.sponsorshipThis article was funded by the Project POCI-01-0145-FEDER -029947 “Chronic wasting disease risk assessment in Portugal” supported by Portuguese Foundation for Science and Technology (FCT)-FEDER-Balcão2020. This work was also supported by the research unit CECAV and AL 4AnimalS under the projects UIDB/CVT/00772/2020 and LA/P/0059/2020; the research unit CITAB under project UIDB/04033/2020 and PhD grant SFRH/BD/146961/2019, all of them funded by the Portuguese Foundation for Science and Technology (FCT).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPEREIRA, Jorge C.[et al.] (2023) - Estimating sequence diversity of prion protein gene (PRNP) in Portuguese populations of two cervid species: red deer and fallow deer. Prion. 17:1, p. 75-81, DOI: https://doi.org/10.1080/19336896.2023.2191540pt_PT
dc.identifier.doi10.1080/19336896.2023.2191540pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.11/8746
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCervidpt_PT
dc.subjectCWDpt_PT
dc.subjectPortugalpt_PT
dc.subjectPrionpt_PT
dc.subjectPRNPpt_PT
dc.subjectSusceptibilitypt_PT
dc.titleEstimating sequence diversity of prion protein gene (PRNP) in Portuguese populations of two cervid species: red deer and fallow deerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage81pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage75pt_PT
oaire.citation.titlePrionpt_PT
oaire.citation.volume17pt_PT
person.familyNameFigueira
person.familyNameMatos
person.familyNameRibeiro Pinto
person.givenNameLuís Manuel Faria
person.givenNameAna
person.givenNameMaria de Lurdes
person.identifier.ciencia-id941B-C381-8158
person.identifier.ciencia-id2711-E07A-FE45
person.identifier.ciencia-idE81F-5A9E-5C8D
person.identifier.orcid0000-0002-8454-2880
person.identifier.orcid0000-0001-9709-862X
person.identifier.orcid0000-0002-5928-6483
person.identifier.ridK-6664-2013
person.identifier.scopus-author-id55626078200
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication74ef6ec4-da08-461e-a861-a4f5beb24858
relation.isAuthorOfPublicationb6aa356b-8fa0-42be-af99-b34818a15cdc
relation.isAuthorOfPublicatione1a553fd-7452-47c4-a87d-f5863dd02746
relation.isAuthorOfPublication.latestForDiscovery74ef6ec4-da08-461e-a861-a4f5beb24858

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