Identification of proteaginous pea cultivars (Pisum sativum L.) using microsatellites molecular markers

Reis, C.M.G.; Diogo, Maria da Graça; Henriques, Nuno

Publication

Identification of proteaginous pea cultivars (Pisum sativum L.) using microsatellites molecular markers

2012Conference object

dc.contributor.author	Reis, C.M.G.
dc.contributor.author	Diogo, Maria da Graça
dc.contributor.author	Henriques, Nuno
dc.date.accessioned	2012-06-05T16:06:19Z
dc.date.available	2012-06-05T16:06:19Z
dc.date.issued	2012
dc.description.abstract	Abstract: Conventionally morphological descriptors are routinely used for establishing the identity of varieties. This kind of descriptors has some disadvantages, namely most of them are quantitative, controlled by several pairs of genes, and their expression is influenced by environmental factors. Molecular markers have a potential to facilitate this procedure, increase the reliability of decisions, and substantially save the time and space needed for experiments. In this study we intended to identify 20 cultivars of proteaginous pea (Pisum sativum L.), registered in the Community Catalog of Varieties, by microsatellites molecular markers. After DNA extraction, seven different loci were analyzed. PCR amplifications were conducted and the resulting fragments were separated on an 3,5% MS-8 agarose gel in TBE buffer, at 90V/h. The gels were analyzed for the presence/ absence of bands and a table with binary code was made. The data were processed with the statistical software NTSYS-pc, using the SIMQUAL module and Jaccard similarity coefficient, followed by UPGMA cluster analysis. With the analysis of six polymorphic loci was possible to distinguish almost all of cultivars. The most informative loci were AD61 and AB53. The cluster analysis of SSR markers separated the pea genotypes into two distinct clusters. The first cluster included the five cultivars: Isard, Cartouche, Audit, Corrent and James. The second cluster included the remaining fifteen cultivars and was further divided in two subclusters. The first subcluster had the Portuguese genotype Grisel and second subcluster contained the remaining fourteen cultivars. In this subcluster Ideal and Alezan had 100 percent similarity. There was a low number of heterozygous loci which is consistent with the nature of self pollinated species. The results showed a high potential and resolving power of SSR markers in distinctness assessment. SSR markers might also be useful in Pisum sativum L. germplasm management and genetic diversity studies.	por
dc.description.sponsorship	The present study was financially by the EU within the POCTEP – “Programa Operacional de Cooperação Transfronteiriça Espanha - Portugal”, Project 0186_AGROCELE_3_E.	por
dc.identifier.citation	REIS, C.M.G. ; DIOGO, M.G. ; HENRIQUES, N. (2012) - Identification of proteaginous pea cultivars (Pisum sativum L.) using microsatellites molecular markers. In Jornadas Portuguesas de Genética, 37, Lisboa, 28-30 de Maio.	por
dc.identifier.uri	http://hdl.handle.net/10400.11/1288
dc.language.iso	eng	por
dc.peerreviewed	yes	por
dc.subject	Pea	por
dc.subject	Pisum sativum
dc.subject	DNA fingerprinting
dc.subject	SSR markers
dc.subject	Cultivar identification
dc.title	Identification of proteaginous pea cultivars (Pisum sativum L.) using microsatellites molecular markers	por
dc.type	conference object
dspace.entity.type	Publication
oaire.citation.conferencePlace	Lisboa	por
oaire.citation.title	XXXVII Jornadas Portuguesas de Genética 2012	por
person.familyName	Gaspar Reis
person.givenName	Carlos
person.identifier.ciencia-id	A71B-FF9D-83C6
person.identifier.orcid	0000-0002-4854-5128
rcaap.rights	openAccess	por
rcaap.type	conferenceObject	por
relation.isAuthorOfPublication	74d754d4-3926-497b-a0c4-26e98cbe0c93
relation.isAuthorOfPublication.latestForDiscovery	74d754d4-3926-497b-a0c4-26e98cbe0c93