ESACB - Pósteres em encontros científicos/técnicos
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- Impact of microalgae incorporation on sensory profile and consumer’s expectations of bread and cheesePublication . Ramos, R.M.; Barbosa, B.; Baião, L.; Lima, R.C.; Rocha, C.; Hernandéz, H.; Mahmoud, N.; Tavares, S.; Carvalho, C.C.C.R.; Nunes, M.C.; Raymundo, A.; Anjos, O.; Cunha, L.M.Microalgae are a promising future food source with their rich nutrient content and potential to contribute to a carbonneutral economy. However, incorporating microalgae into food formulations presents sensory challenges, particularly in terms of colour and distinct {sh-like aromas. In the YUM ALGAE project, this study successfully evaluated the impact of microalgae incorporation in two distinct food matrices – bread and camembert-like cheese. Four loaf bread samples and cheeses were assessed, incorporating chlorella (Chlorella vulgaris) or spirulina (Arthrospira platensis), both raw and with enzymatic treatment. A panel of 65 naïve tasters (aged between 20-59 years) was {rst invited to evaluate all the products’ expectations on a 9-point hedonic scale, visualising half-loafs and half-cheeses on a simulated store shelf. Then, at the sensory booth, participants were instructed to evaluate the samples’ appearance and odour, rating their overall acceptance and indicating the aspects they valued most and least when presented sequentially with a slice of each product. Simultaneously, using a Rate-All-That-Apply (RATA) ballot for appearance (no. of attributes: bread-11 and cheeses-8) and odour (no. of attributes: bread-10 and cheeses-8), a trained panel (n=10) evaluated each of the samples. The attributes were pre-de{ned based on the team experience, a literature review, and a pilot test with dried biomass from microalgae. Based on the naïve tasters' evaluation, all the samples had a high mean acceptance score (>6.0). No signi{cant differences were found between samples with raw microalgae incorporation and those treated with enzymes, except for cheese with chlorella, where the sample with raw chlorella had a higher value (p=0.02). Regarding the sensory pro{le, the sea-like odour intensi{es, and the cheese/bread odour decreases for the samples with enzymatic treatment. This research highlights the success of sensory research in the development of new alternative proteins, instilling con{dence in the potential to overcome the associated sensory barriers.
- Differentiation of honey produced in two PDO regions in Portugal using FTIRT-ATR analysisPublication . Pedro, Soraia; Ferreira, Ana; Gouveia, Cecília; Gallardo, Eugenia; Rodrigues, A.M.; Peres, Maria de Fátima; Anjos, O.The chemical composition of honey in a Protected Designation of Origin (PDO) reflectes the unique characteristics of the region, ensuring authenticity through rigorous standards. To identify the consistent analytical parameters within a particular PDO and assess the possibility of distinguishing them using rapid techniques, such as FTIR-ATF (Fourier Transform Infrared Spectroscopy with Attenuated Total Reflectance).
- Melissopallinology characterization of Portuguese honeys with protected designation of originPublication . Almeida, Paulo Ruddo; Lima, Irailde; Anjos, O.; Paulo, Luísa; Vilas-Boas, MiguelThe European Union created, through Regulations No. 2081/92 and No. 2082/92, Protected Designation of Origin (PDO) as an instrument for valuing agricultural products with distinctive particularities, demanding for this purpose the establishment of requirements set out in a specification book.
- Assessing beekeeping potential in a Portuguese area with honey Protected Designation of OriginPublication . Silveira, Carlos; Roque, Natália; Fernandez, Paulo; Anjos, O.; Vilas-Boas, MiguelThis study aims to assess the beekeeping potential in a Portuguese area with honey protected designation of origin (PDO) following a multi-criteria decision analysis (MCDA) for supporting beekeepers in the selection of the best locations for apiaries, thus maximizing honey production and reducing the risk of bee colony losses.
- A preliminar study on Brucella spp. seroprevalence in wild boar (Sus scrofa) in PortugalPublication . Pires, Humberto; Matos, Manuela; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Pintado, Cristina; Santos Pintado, Cristina Maria Baptista; Figueira, Luis; Figueira, Luís Manuel Faria; Mesquita, João R.; Matos, Ana Cristina; Matos, Ana; Coelho, Ana CláudiaVarious species of the genus Brucella are highly virulent zoonotic agents. Brucella melitensis, B. abortus, and B. suis area broadly spread worldwide and animal brucellosis has a significant economic impact. This zoonotic disease affects both domestic pigs and wild pigs such as wild boar (Sus scrofa) In order to investigate the importance of wild boar (Sus scrofa) in the epidemiology of Brucella infection in Portugal, a serological survey was performed on samples from 184 wild boar fron the Centre region of Portugal.
- A serological survey of Brucella spp. in red deer (Cervus elaphus) in PortugalPublication . Pires, Humberto; Matos, Manuela; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Santos Pintado, Cristina Maria Baptista; Figueira, Luís Manuel Faria; Mesquita, João R.; Matos, Ana; Coelho, Ana CláudiaWildlife has been identified as important sentinels for the surveillance of zoonotic pathogens such Brucella infection. Systematic brucellosis monitoring in wildlife is not demanded by regulatory acts, but several studies have reported the presence of this infection in European countries. In order to investigate the importance of red deer (Cervus elaphus) in the epidemiology of Brucella infection in Portugal, a serological survey was performed on samples of 276 hunted red deer from the centre of Portugal.
- Evidence for sporadic Coxiella burnetii excretion in sheep milk, central PortugalPublication . Pires, Humberto; Silva, S. Santos; Cruz, A.; Cardoso, Luís; Lopes, A.P.; Pereira, M.A.; Nóbrega, C.; Mega, A.C.; Santos, C.; Cruz, R.; Esteves, F.; Vala, H.; Matos, Ana; Barradas, P.F.; Coelho, A.C.; Mesquita, J.R.Coxiella burnetii, the causative agent of Q fever, is a globally distributed zoonosis, with cattle, sheep, and goats considered the main reservoirs of the disease (Agger et al,., 2013; Bento et al., 2023). Human transmission primarily occurs through inhaling infections aerosols from milk, faeces, urina, and birth products from infected ruminants. This study used a 2-year longitudinal approach to investigate C. burnetii excretion in bulk tank milk samp+les from sheep in central Portugal.
- Seroprevalence of West Nile virus antibodies in wild birds of prey in PortugalPublication . Loureiro, Filipa; Cardoso, Luis; Matos, Ana; Matos, Manuela; Silva, Filipe; Coelho, Ana CláudiaWest Nile Virus is one of the most widely distributed arboviruses in the world with zoonotic potential. Seroepideiological studies are necessary to establish proper prevention and control strategies. WNV-total antibodies were searched in blood serum using an ELISA commercial kit. Samples from 136 individuals of diurnal raptors were analysed, from eight distinct Accipitriformes species. Eleven nocturnal birds of prey, belonging to four differente species of Strigiformes were tested. In this study, 47samples were positive (34.56%; 95% CI: 26.62-43.19%).
- Seroprevalence of West Nile virus in Cinereous vultures (Aegypius monachus) and Griffon vultures (Gyps fulvus) in the centre region of PortugalPublication . Loureiro, Filipa; Cardoso, Luis; Matos, Ana Cristina; Matos, Ana; Matos, Manuela; Silva, Filipe; Coelho, Ana CláudiaWest Nine virus (WNV) is an emergent zoonotic pathogen spread worldwide. This flavivirus is endemically maintained in a life cycle with birds acting as reservoirs, Culex mosquitopes as the main vectors, and human and horses as accidental dead-end hosts. The potential impact ofWNV infection on wildlife populations can be severe, especially in thjreatened or endangered species. This work might be useful as a starting point for a surveillance program to better assess the real distribution of the WNV in Portuguese vulutres. Early virus detection is important for both bird conservation and public health.
- Seroprevalence of West Nile Virus (WNV) in birds in European countries: a systematic reviewPublication . Loureiro, Filipa; Cardoso, Luis; Matos, Manuela; Coelho, Ana Cláudia; Matos, Ana