Honey sugars analysis by ion chromatography method with Integrated Pulsed Amperometric Detection (IPAD)

Antunes, Paulo; Paulo, Luísa; Barbosa, Ana; Anjos, O.

Publication

Honey sugars analysis by ion chromatography method with Integrated Pulsed Amperometric Detection (IPAD)

2012Conference object

dc.contributor.author	Antunes, Paulo
dc.contributor.author	Paulo, Luísa
dc.contributor.author	Barbosa, Ana
dc.contributor.author	Anjos, O.
dc.date.accessioned	2012-07-27T17:05:37Z
dc.date.available	2012-07-27T17:05:37Z
dc.date.issued	2012
dc.description.abstract	Glucose and fructose are the most important monosaccharides in honey, these sugars combined in various forms comprise the di- and trisaccharide fractions of floral honey. Frutose/glucose ratio can influence the flavor of honey since fructose is sweeter than glucose. Honeys with higher fructose/glucose ratios remain liquid for longer periods. Several Chromatographic methods have been used to evaluate the sugar content of honey, although the Integrated Pulsed Amperometric Detection (IPAD) only recently became to be used. In order to characterize the sugar content in honey from different botanical origins a Dionex ICS3000 ion chromatograph was used. Separation was performed in a column “CarboPacTM PA20 3x150mm”, with a precolumn “CarboPacTM PA20 3x30mm”. Electrochemical detector in Integrated Pulsed Amperometric Detection (IPAD) mode was used. The elution was performed with a gradient with two NaOH solutions (10 and 200 mM). Standard solutions of glucose, fructose and sucrose, were used to identify and quantify the individual sugar components in the honey samples. The method showed a good separation between the compounds (resolution> 3.3 and more than 9000 theoretical plates). The response was linear in the range (0.05 to 1.00 mg/L for fructose and glucose and 0.01 to 0.20 mg/L for sucrose). The detection limits were 0.014 mg/L for glucose, 0.007 mg/L for fructose and 0.003 mg/L for sucrose. Twenty six monofloral honey samples from Erica, Citrus, Lavandula and Eucalyptus were tested. 5,000 g of each honey sample was diluted to 1 mg/L and filtered with a 0.45 m polypropylene filter prior to HPLC analysis. The results show that the fructose/glucose ratios are in accordance with the different honeys analyzed and the HPLC-IPAD is a good methodology to determine the sugar content in honey, with low solvent consumption and residues.	por
dc.identifier.citation	ANTUNES, P. [et al.] (2012) - Honey sugars analysis by ion chromatography method with Integrated Pulsed Amperometric Detection (IPAD). In Encontro Nacional de Cromatografia, 7 = National Meeting on Chromatography, 7th, Porto, 9-11 de Janeiro - Book of abstracts. Porto : FCUP. pp146.	por
dc.identifier.uri	http://hdl.handle.net/10400.11/1389
dc.language.iso	eng	por
dc.subject	Honey sugars	por
dc.subject	IPAD	por
dc.title	Honey sugars analysis by ion chromatography method with Integrated Pulsed Amperometric Detection (IPAD)	por
dc.type	conference object
dspace.entity.type	Publication
oaire.citation.conferencePlace	Porto	por
oaire.citation.title	7.º Encontro Nacional de Cromatografia, Poro, 9-11 de janieor de 2012	por
person.familyName	Anjos
person.givenName	Ofélia
person.identifier.ciencia-id	C21D-D8C7-3037
person.identifier.orcid	0000-0003-0267-3252
person.identifier.rid	G-2808-2012
person.identifier.scopus-author-id	23395659700
rcaap.rights	openAccess	por
rcaap.type	conferenceObject	por
relation.isAuthorOfPublication	df9191ae-0bbb-4bb8-bbdc-0f79c7365876
relation.isAuthorOfPublication.latestForDiscovery	df9191ae-0bbb-4bb8-bbdc-0f79c7365876