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Assessment of Genetic Diversity in Opuntia spp. Portuguese Populations Using SSR Molecular Markers

2018Journal article Open access
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dc.contributor.authorReis, C.M.G.
dc.contributor.authorRaimundo, Joana
dc.contributor.authorRibeiro, M.M.A.
dc.date.accessioned2018-05-01T22:58:11Z
dc.date.available2018-05-01T22:58:11Z
dc.date.issued2018
dc.description.abstractThe Opuntia spp., most likely few individuals, were introduced in the Iberian Peninsula in the beginning of the 16th century, after the discovery of America, spreading afterwards throughout the Mediterranean basin. We analysed, for the first time, the genetic diversity in a set of 19 Portuguese Opuntia spp. populations from the species O. ficus-indica, O. elata, O. dillenii and O. robusta using nuclear microsatellite (nuSSR) markers. The Italian cultivars ‘Bianca’, ‘Gialla’ and ‘Rossa’ were included in the study for comparison purposes. The nuSSR amplifications produced from five to 16 alleles, with an average of 9.2 alleles per primer pair, and average polymorphism information content of 0.71. The estimated Dice coefficient among populations varied from 0.26 to 1.0, indicating high interspecific genetic diversity but low genetic diversity at the intraspecific level. The hierarchical clustering analysis revealed four major groups that clearly separated the four Opuntia species. Among the O. ficus-indica populations, two sub-clusters were found, one including the white pulp fruits (with cv. Bianca) and the other with the orange pulp ones and including the cv. Gialla, the cv. Rossa, and one pale yellow pulp population. No genetic differences were found between the inermis form, O. ficus-indica f. ficus-indica, and the rewilded spiny one, O. ficus-indica f. amyclaea. The dendrogram indicated that the clustering pattern was unrelated to geographical origin. The results revealed a low level of genetic diversity among the Portuguese populations of O. ficus-indica.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationReis, C.M.G.; Raimundo, J.; Ribeiro, M.M.A. Assessment of Genetic Diversity in Opuntia spp. Portuguese Populations Using SSR Molecular Markers. Agronomy 2018, 8, 55-65.pt_PT
dc.identifier.doi10.3390/agronomy8040055pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.11/6066
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPI AG, Basel, Switzerlandpt_PT
dc.relation.publisherversionhttp://www.mdpi.com/2073-4395/8/4/55pt_PT
dc.subjectCactus pearpt_PT
dc.subjectCluster analysispt_PT
dc.subjectDNA fingerprintingpt_PT
dc.subjectMicrosatellite markerspt_PT
dc.subjectPrincipal coordinate analysispt_PT
dc.titleAssessment of Genetic Diversity in Opuntia spp. Portuguese Populations Using SSR Molecular Markerspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F00681%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F00239%2F2013/PT
oaire.citation.endPage65pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage55pt_PT
oaire.citation.titleAgronomypt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameGaspar Reis
person.familyNameRaimundo
person.familyNameRibeiro
person.givenNameCarlos
person.givenNameJoana
person.givenNameMaria Margarida
person.identifier349097
person.identifier.ciencia-idA71B-FF9D-83C6
person.identifier.ciencia-idAD12-4D32-7A48
person.identifier.orcid0000-0002-4854-5128
person.identifier.orcid0000-0002-0850-4401
person.identifier.orcid0000-0003-4684-1262
person.identifier.ridM-4235-2013
person.identifier.scopus-author-id7201715611
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublicationa456aa66-5c6e-41f0-9501-38ff9e629ac7
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relation.isProjectOfPublication0dd6ac36-59d6-49b9-af6f-8d07c8a3dedc
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