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Reproductive toxicity assessment of four portuguese plant hydrolates: Effects on oocyte maturation and sperm viability

2025Research article Open access
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dc.contributor.authorDias, Sandra D.F.
dc.contributor.authorAndrade, L.P.
dc.contributor.authorRolo, Joana
dc.contributor.authorGaspar, Carlos
dc.contributor.authorRuivo, Patrícia Gomes
dc.contributor.authorOliveira, Ana Sofia
dc.contributor.authorFerreira, Sandra Saraiva
dc.contributor.authorOliveira, Rita Palmeira
dc.contributor.authorOliveira, José Martinez de
dc.contributor.authorGonçalves, José Carlos
dc.contributor.authorDelgado, F.M.G.
dc.contributor.authorOliveira, Ana Palmeira de
dc.date.accessioned2025-10-24T15:58:58Z
dc.date.available2025-10-24T15:58:58Z
dc.date.issued2025
dc.date.updated2025-10-21T16:16:44Z
dc.descriptionEthical review and approval were waived for this study due to the use of materials that were collected at the slaughterhouse and were going to be discarded. The study design is in compliance with an established protocol between Universidade da Beira Interior (Covilhã, Portugal) and the slaughterhouse “OVIGER” (Alcains, Portugal).
dc.description.abstractExtracts of aromatic medicinal plants have been extensively studied regarding their numerous bioactivities. However, despite being highly used by humans, studies on the safety of these extracts for animal use are scarce. In this study, we aim to contribute to the determination of the safety profile of plant extracts by focusing on the reproductive toxicity of hydrolates (a by-product of essential oils production) of four endogenous Portuguese plants—Cistus ladanifer, Cupressus lusitanica, Helychrisium italicum, and Thymbra capitata—by studying their effects on bovine oocytes and spermatozoa. To achieve our aims,we determined the oocyte maturation and viability rate in the bovine in vitro maturation test (bIVM) and the bovine sperm viability using the eosin–nigrosin test, in the presence of five concentrations of each hydrolate at half-log intervals (% v/v of culture media). We found that hydrolates did not affect oocyte maturation or viability (maximum concentration tested: 0.2%, v/v). Regarding the sperm viability test, we found that T. capitata and C. ladanifer hydrolates impaired sperm viability (p < 0.05) (maximum concentration tested: 0.2%, v/v), in comparison with the negative control. In summary, we found that H. italicum and C. lusitanica hydrolates were safe regarding oocyte maturation, oocyte viability, and sperm viability, being candidates to be included in bovine husbandry as feeding additives.eng
dc.description.sponsorshipThis work was supported by the “INOVEP project—Innovation with Plant Extracts”, I&DT projects for companies in collaboration with scientific entities, project number 33815, Centro2020. It was also funded by the Foundation for Science and Technology (FCT), through funds from the State Budget, and by the European Regional Development Fund (ERDF), under the Portugal 2020 Program, through the Regional Operational Program of the Center (Centro2020), through the Project with the reference UIDB/00709/2020. Financial support was also provided by FCT through a PhD fellowship (SFRH//BD/136192/2018) awarded to ASO, and a postdoctoral fellowship awarded to JR (SFRH/BPD/115145/2016), and by the Portuguese Foundation for Science and Technology through the project UIDB/00212/2020, and FCT—Fundação para a Ciência e Tecnologia, I.P., through the CERNAS project references UIDB/00681/2020 (DOI: 10.54499/UIDB/00681/2020).
dc.description.versionN/A
dc.identifier.citationDIAS, Sandra D.F. [et al.] (2025) - Reproductive toxicity assessment of four portuguese plant hydrolates: Effects on oocyte maturation and sperm viability. Animals. DOI: 10.3390/ani15192838
dc.identifier.doi10.3390/ani15192838en_US
dc.identifier.slugcv-prod-4584732
dc.identifier.urihttp://hdl.handle.net/10400.11/10340
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationUIDP/00681/2020en_US
dc.relationDOI 10.54499/UIDP/00681/2020en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCistus ladanifer
dc.subjectCupressus lusitanica
dc.subjectHelychrisium italicum
dc.subjectOocyte
dc.subjectReproductive toxicity
dc.subjectSpermatozoa
dc.subjectThymbra capitata
dc.subjectViability
dc.titleReproductive toxicity assessment of four portuguese plant hydrolates: Effects on oocyte maturation and sperm viabilityeng
dc.typeresearch articleen_US
dspace.entity.typePublication
oaire.citation.titleAnimalsen_US
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameDuarte da Fonseca Dias
person.familyNameMota Pinto de Andrade
person.familyNameGonçalves
person.familyNameDelgado
person.givenNameSandra
person.givenNameLuís Pedro
person.givenNameJosé
person.givenNameF.M.G.
person.identifier508085
person.identifier.ciencia-id6E19-689C-5336
person.identifier.ciencia-idDA1F-0C25-23F1
person.identifier.ciencia-idAE1F-12BE-65DD
person.identifier.ciencia-idC715-76B6-A07D
person.identifier.orcid0000-0003-0123-8400
person.identifier.orcid0000-0002-9206-2350
person.identifier.orcid0000-0003-3387-4487
person.identifier.orcid0000-0003-1238-9386
person.identifier.scopus-author-id7103325479
rcaap.cv.cienciaidC715-76B6-A07D | Fernanda Maria Grácio Delgado Ferreira de Sousa
rcaap.rightsopenAccessen_US
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relation.isAuthorOfPublication2bb821ef-7e5a-4a7a-9064-45b5fc394432
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relation.isAuthorOfPublication.latestForDiscoverya22b0ac5-c026-40e7-aebc-0dff1f54fa4b

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