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Molecular and serological screening support the lack of Coxiella burnetii circulation in wild birds of Portugal

2025Research article Open access
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dc.contributor.authorLoureiro, Filipa
dc.contributor.authorMesquita, João R.
dc.contributor.authorCardoso, Luis
dc.contributor.authorPintado, Cristina Santos
dc.contributor.authorGonçalves, S. Gomes
dc.contributor.authorMatos, Ana
dc.contributor.authorSoeiro, V.
dc.contributor.authorGonçalves, A.
dc.contributor.authorSilva, Filipe
dc.contributor.authorMatos, Manuela
dc.contributor.authorCoelho, Ana Cláudia
dc.date.accessioned2025-03-20T15:45:57Z
dc.date.available2025-03-20T15:45:57Z
dc.date.issued2025
dc.description.abstractCoxiella burnetii is a highly infectious zoonotic pathogenic bacterium that has a major economic impact in the livestock industry throughout the world and causes unpredictable outbreaks in humans worldwide. Although it is known that birds are potential reservoirs of C. burnetii, their role in the epidemiological cycle of the pathogen has not been fully verified. Due to its non-specific symptoms and clinical signs, it is certainly an underdiagnosed disease. The objective of this study was to obtain more information on C. burnetii prevalence in wild birds in Portugal. Blood, plasma, and other tissue samples were obtained from wild birds admitted at wildlife rehabilitation centres in Portugal in the scope of passive surveillance. Antibodies specific to C. burnetii were screened using a commercial enzyme-linked immunosorbent assay according to the manufacturer’s instructions. Evidence of C. burnetii infection was sought based on the detection of bacterial DNA. No positive results were found, either in terms of antibodies to C. burnetii or molecular biology. These serological findings do not indicate the endemic circulation of C. burnetii in wild birds, which can be considered relevant information. However, a more complete and serialized approach over time is necessary to be able to make real inferences about the endemicity of the pathogen in the country and its dispersion among wild avian populations. qPCR results were also negative, a finding suggesting that this host population may not play a significant role in the transmission dynamics of C. burnetii. Given the importance of wild bird species as natural reservoirs of this zoonotic bacterium, we consider these data useful for multidisciplinary work in the prevention and control of Q fever, following a One Health approach.eng
dc.description.sponsorshipThe authors would like to thank the teams at CRAS-HVUTAD, CRASSA, and PBG for their cooperation in collecting the samples. A huge thank you to the Laboratory of Microbiology of the School of Agriculture, Polytechnic University of Castelo Branco, and to the Microbiology and Infectious Diseases Laboratory of the School of Medicine and Biomedical Sciences (ICBAS), University of Porto.
dc.identifier.citationLOUREIRO, Filipe [et al.] (2025) - Molecular and serological screening support the lack of Coxiella burnetii circulation in wild birds of Portugal. Birds. 6:3, 12 p. DOI: doi.org/10.3390/birds6010003
dc.identifier.doihttps://doi.org/10.3390/birds6010003
dc.identifier.urihttp://hdl.handle.net/10400.11/10074
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAvian
dc.subjectGenotyping
dc.subjectOne Health
dc.subjectPublic health
dc.subjectQ fever
dc.subjectPCR
dc.subjectReservoirs
dc.subjectSeroprevalence
dc.subjectZoonosis
dc.titleMolecular and serological screening support the lack of Coxiella burnetii circulation in wild birds of Portugaleng
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issue3
oaire.citation.startPage3
oaire.citation.titleBirds
oaire.citation.volume6
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameMatos
person.givenNameAna
person.identifier.ciencia-id2711-E07A-FE45
person.identifier.orcid0000-0001-9709-862X
person.identifier.ridK-6664-2013
person.identifier.scopus-author-id55626078200
relation.isAuthorOfPublicationb6aa356b-8fa0-42be-af99-b34818a15cdc
relation.isAuthorOfPublication.latestForDiscoveryb6aa356b-8fa0-42be-af99-b34818a15cdc

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