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Authors
Advisor(s)
Abstract(s)
The Pterospartum tridentatum (L.) Willk is widely used as medicinal plant
for the treatment of digestive tract diseases and diabetes and for culinary
purposes, however, characterization studies on this plant extracts are scarce.
This work aimed to develop methodologies for micropropagation of
this species and carry out characterization and application of the aqueous
extracts. This study represents the first comparison analysis of extracts obtained
from wild plants and aerial parts of plants in vitro multiplication. Micropropagation was very effective on large scale propagation with
high multiplication rates.
Aqueous extracts showed a good performance, high levels of phenolic
compounds and antioxidant activity both in wild plants and in micropropagation.
Phenolic profiles in in vitro shoots are very similar to extracts of
wild plants during vegetative rest.
The extracts revealed no antifungal activity, but nevertheless evidenced
inhibition for some microorganisms.
Within the monosaccharides, glucose is the predominant sugar in wild
plants, since in the in vitro shoots uronic acids are the main components.
An interesting result was the higher molar percentages of rhamnose and
uronic acids detected on in vitro shoots compared to wild plants, which
can make the in vitro plant material very useful to obtain these compounds.
The application of chitosan bioactive coatings incorporating extracts
has improved post-harvest storage capacity in cherry. The results allow propose
these methodologies for obtaining extracts with bioactive properties
from micropropagated material without depleting natural resources.
Description
Keywords
Pterospartum tridentatum Propagação in vitro
Pedagogical Context
Citation
COELHO, M.T. (2016) - Estudos de propagação in vitro, caracterização e valorização de carqueja (Pterospartum tridentatum (L.) Willk). In III Ciclo de Conferências : Conselho Técnico-Científico : temas atuais em investigação. Castelo Branco : IPCB, 2016. ISBN 978-989-8196-57-6. p. 39-44.