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Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis
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Advisor(s)
Abstract(s)
The purpose of this paper was to optimize the production of simple sugars from olive
grove pruning (OGP) using acid pretreatment and enzymatic hydrolysis. This study was based on
a model composition corresponding to a 34 orthogonal factorial design and employed the
response surface methodology (RSM) to optimize the pretreatment and hydrolysis conditions,
aiming to attain maximum glucose, xylose and arabinose extraction from cellulose and
hemicellulose of biomass. The pretreatment operating conditions considered for optimization,
were temperature (60–180 °C), residence time (30–120 min) and sulphuric acid concentration
(0.5–5% w w-1). Enzymatic hydrolysis experiments on solid fraction pretreated with diluted acid
were performed at a solid concentration of 5% (w v-1, based on dry weight), using 50 mM citrate
buffer pH 4.8 with BSA at a concentration of 60 mg g-1 dry biomass. The reaction mixture was
incubated at 50 °C for 174 h on an orbital shaker at 150 rpm. Three commercial enzyme
preparations (cellulase complex, b-glucosidase and xylanase) were used in enzymatic
saccharification. Total carbohydrate content of the initial biomass was 51.25% (in dry mass), of
which glucose was the major constituent with 33.59%. Contents of lignin and extractable found
in biomass were 24.96% and 15.84%, respectively. In this work, it was possible to extract 93.1%
of the sugars present in the olive grove pruning, with pretreatments carried out for 102 min at
156 °C with a sulfuric acid load of 4.09% (w w-1), followed by enzymatic hydrolysis performed
for 174 h, with an enzyme loading of 18 PFU, 36 p-NPGU and 36 IU per gram of substrate.
Description
Keywords
Acid hydrolysis Enzymatic hydrolysis Olive pruning Pretreatment RSM Sugars
Pedagogical Context
Citation
PEDRO, Nuno [et al.] (2024) - Production of simple sugars from olive grove pruning using acid pretreatment and enzymatic hydrolysis. Agronomy Research. 22:2, p. 876-886. DOI: 10.15159/AR.24.069
Publisher
Agronomy Reserach