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Humulus lupulus aqueous extract and hydrolate as a potential ingredient for cosmetics : chemical characterization and in vitro antimicrobial, cytotoxicity, antioxidant, and anti-inflammatory assessment

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dc.contributor.authorValente, João Vasco
dc.contributor.authorOliveira, Rita Palmeira de
dc.contributor.authorGuiomar, Liliana
dc.contributor.authorVaz, Cátia
dc.contributor.authorRolo, Joana
dc.contributor.authorGaspar, Carlos
dc.contributor.authorOliveira, Ana Sofia
dc.contributor.authorCaramelo, Débora
dc.contributor.authorBreitenfeld, Luísa
dc.contributor.authorGonçalves, José Carlos
dc.contributor.authorDelgado, F.M.G.
dc.contributor.authorOliveira, José Martinez
dc.contributor.authorOliveira, Ana Palmeira de
dc.date.accessioned2024-02-16T16:35:01Z
dc.date.available2024-02-16T16:35:01Z
dc.date.issued2024
dc.date.updated2024-02-15T17:20:01Z
dc.description.abstractHumulus lupulus extracts have in their composition different molecules, such aspolyphenols, α-acids, β -acids, and hydrocarbons, which contribute to the plant’s medicinal properties. These molecules are associated with antimicrobial, antioxidant, and anti - inflammatory activities. OBJECTIVE This work focuses on the evaluation of H. lupulus biological activities, with the aim of evaluating its potential for inclusion in cosmetic formulations. METHODS Two distinct aqueous extracts and two hydrolates obtained via hydrodistillation were evaluated. These include the flower parts (FE, FH) and the mix of aboveground parts (ME, MH). The chemical profiles for both aqueous extracts and hydrolates were identified by high performance liquid chromatography (HPLC chromatography -mass spectrometry (GC -MS ). Antimicrobial, antioxidant, cytotoxicity , and anti-inflammatory activity were tested in vitro using standard methods. RESULTS Rutin was the major compound found in FE (40.041 µg mg -1 of extract) and ME (2.909 µg mg -1 of extract), while humulenol II and was the most abundant compound in hydrolates (FH:20.83%; MH: 46.80%). Furthermore, FE was able to inhibit the growth of Staphylococcus aureus and Staphylococcus epidermis with MIC values of 50% and 25% (v/v), respectively. FH showed the same effect Staphylococcus aureus (50% v/v). FH evidenced poor antioxidant potential in DPPH scavenging test, it demonstrated significant antioxidant and anti-inflammatory effects by reducing (***p<0.001) intracellular reactive oxygen species (ROS), NO (nitric oxide) levels (***p<0.001) and cyclooxygenase -2 (COX-2) protein expression (***p<0.001) in lipopolysaccharide (LPS) -stimulated macrophages. Nevertheless, it is important to note that FH exhibited cytotoxicity at high concentrations in 3T3 fibroblasts and RAW macrophages. CONCLUSION The studied H. lupulus aqueous extracts and hydrolates revealed that FH stands out as the most promising bioactive source for cosmetic formulations. However, future research addressing antimicrobial activity is necessary to confirm its potential incorporation into dermatological and cosmetic formulations.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationVALENTE, João [et al.] (2023) - Humulus lupulus aqueous extract and hydrolate as a potential ingredient for cosmetics : chemical characterization and in vitro antimicrobial, cytotoxicity, antioxidant, and anti-inflammatory assessment. Fitoterapia. DOI: 10.1016/j.fitote.2024.105861pt_PT
dc.identifier.doi10.1016/j.fitote.2024.105861pt_PT
dc.identifier.issn0367-326X
dc.identifier.slugcv-prod-3816715
dc.identifier.urihttp://hdl.handle.net/10400.11/8882
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationUID/AMB/00681/2013pt_PT
dc.subjectAnti -inflammatorypt_PT
dc.subjectCosmeticspt_PT
dc.subjectPlant-based compoundspt_PT
dc.subjectWound healingpt_PT
dc.titleHumulus lupulus aqueous extract and hydrolate as a potential ingredient for cosmetics : chemical characterization and in vitro antimicrobial, cytotoxicity, antioxidant, and anti-inflammatory assessmentpt_PT
dc.typepreprint
dspace.entity.typePublication
oaire.citation.startPage105861pt_PT
oaire.citation.titleFitoterapiapt_PT
person.familyNameCaramelo
person.familyNameGonçalves
person.familyNameDelgado
person.givenNameDébora
person.givenNameJosé
person.givenNameF.M.G.
person.identifier1959038
person.identifier508085
person.identifier.ciencia-idEA1D-EF0D-F6B7
person.identifier.ciencia-idAE1F-12BE-65DD
person.identifier.ciencia-idC715-76B6-A07D
person.identifier.orcid0000-0002-6135-9645
person.identifier.orcid0000-0003-3387-4487
person.identifier.orcid0000-0003-1238-9386
person.identifier.scopus-author-id57210150152
person.identifier.scopus-author-id7103325479
rcaap.cv.cienciaidC715-76B6-A07D | Fernanda Delgado
rcaap.rightsrestrictedAccesspt_PT
rcaap.typepreprintpt_PT
relation.isAuthorOfPublicationaed78127-f9c7-44bd-b407-cb2101a6b97c
relation.isAuthorOfPublication3f401604-ccbb-45f3-8ee1-05063cf3d60a
relation.isAuthorOfPublication8ae072f8-0aa1-46ce-830a-210c42f9e11b
relation.isAuthorOfPublication.latestForDiscoveryaed78127-f9c7-44bd-b407-cb2101a6b97c

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