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Genetic variability in wild and domestic populations of Inga edulis Mart. (Fabaceae) in Peruvian Amazon

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dc.contributor.authorRollo, Alexandr
dc.contributor.authorRibeiro, M.M.A.
dc.contributor.authorLojka, Bohdan
dc.contributor.authorHonys, David
dc.contributor.authorSánchez Diáz, Henri Dante
dc.contributor.authorChia Wong, Julio Alberto
dc.contributor.authorVebrová, Hana
dc.contributor.authorCosta, Rita
dc.date.accessioned2012-12-14T17:34:23Z
dc.date.available2012-12-14T17:34:23Z
dc.date.issued2012
dc.description.abstractHuman activity in the Peruvian Amazon causes native vegetation fragmentation into smaller units resulting on the increase of agricultural systems. Understanding the level, the structure and the origin of morphologic within and among populations variation is essential for planning better management strategies aimed at sustainable use and conservation of Inga edulis Mart. species. We evaluated the genetic variability in wild and domestic population to unfold cultivation changes over the species genetic resources. We have studied 400 adult trees: 200 cultivated on arable land and 200 wild growing in untouched lowland rain forest. The individuals were randomly selected. Sampling sites were selected and defined on the basis of the geographical coordinates: longitude, latitude and altitude. Phenotypic variation was monitored using the proposed descriptor of qualitative and quantitative features (e.g., weight of hundred seeds). For each individual a voucher specimen was kept. The total genomic DNA was extracted from young leaves, conserved in silica gel, with INVITEK, Invisorb ®Spin Plant Mini Kit. Samples were then genotyped with five microsatellite (SSR) loci. One locus (Pel5) was cross-transferred, developed previously for Pithecellobium elegans. The remaining four loci (Inga03, 05, 08, 33) were previously developed for the species. Polymerase chain reaction (PCR) was made using a Biometra® T1 Thermocycler using the following profile: 95 °C for 2 min; 95°C for 15 s, 55/59 °C for 30 s, 72 °C for 30 s, 30 cycles; 72 °C for 15 min. The PCR products were fluorescently labelled. The visualization of fragments was carried out according to standard protocols on genetic analyser, ABI PRISM® 310 (Applied Biosystems), using ABI GENESCAN and GENOTYPER software. The phenotypic and genotypic results of wild versus domestic populations are under evaluation to verify if cultivation is altering the allelic variation considering that morphology is considerably changed.por
dc.description.sponsorshipCzech Development Cooperation Project entitled “Sustainable use of natural resources in Peruvian Amazon” Project No. 23/MZe/B/07-10; The Academy of Science of The Czech Republic and the National Council for Science, Technology and Technological Innovation, Peru, binational project entitled “Morphological and genetic diversity of indigenous tropical trees in the Amazon – model study of Inga edulis Mart. in Peruvian Amazon”; Foundation “Nadace Nadání Josefa, Marie a Zdeňky Hlávkových”, Czech Republicpor
dc.identifier.citationROLLO, A. [et al.] (2012) - Genetic variability in wild and domestic populations of Inga edulis Mart. (Fabaceae) in Peruvian Amazon. In International Research on Food Security, Natural Resources on Food Security, and Rural Development, Tropentag, Göttingen, 19-21 September - Resilience of agricultural systems against crisis. Poster.por
dc.identifier.urihttp://hdl.handle.net/10400.11/1584
dc.language.isoengpor
dc.peerreviewedyespor
dc.subjectPeruvian Amazonpor
dc.subjectNative vegetationpor
dc.subjectVariationpor
dc.subjectPopulationpor
dc.subjectInga edulis Mart.por
dc.subjectDNApor
dc.subjectPCRpor
dc.subjectMicrosatellite locuspor
dc.titleGenetic variability in wild and domestic populations of Inga edulis Mart. (Fabaceae) in Peruvian Amazonpor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceTropentag, Göttingenpor
oaire.citation.titleResources on Food Security, and Rural Development, Tropentag, Göttingen, 19-21 September 2012por
person.familyNameRollo
person.familyNameRibeiro
person.familyNameLojka
person.familyNameCosta
person.givenNameAlexandr
person.givenNameMaria Margarida
person.givenNameBohdan
person.givenNameRita
person.identifier349097
person.identifier.ciencia-idAD12-4D32-7A48
person.identifier.ciencia-id8219-DFF3-929F
person.identifier.orcid0000-0002-9258-9144
person.identifier.orcid0000-0003-4684-1262
person.identifier.orcid0000-0002-5059-1975
person.identifier.orcid0000-0002-6975-7576
person.identifier.ridM-4235-2013
person.identifier.ridO-6131-2016
person.identifier.ridO-9136-2018
person.identifier.scopus-author-id7201715611
person.identifier.scopus-author-id23028101300
person.identifier.scopus-author-id57097731700
rcaap.rightsopenAccesspor
rcaap.typeconferenceObjectpor
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relation.isAuthorOfPublication.latestForDiscovery36513d0f-cf84-4fdf-8af0-02d3ea27406c

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