Browsing by Author "Cardoso, Luis"
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- Brucella spp. seroprevalence in wild ungulates in PortugalPublication . Pires, Humberto; Matos, Manuela; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Santos Pintado, Cristina Maria Baptista; Figueira, Luís Manuel Faria; Mesquita, João R.; Matos, Ana; Coelho, Ana CláudiaBrucella spp. seroprevalence in wild ungulates in Portugal.
- Detection of Bagaza virus in Europe: A scoping reviewPublication . Loureiro, Filipa; Mesquita, João R.; Cardoso, Luis; Matos, Ana; Matos, Manuela; Coelho, Ana CláudiaThe Bagaza virus (BAGV) belongs to the genus Orthoflavivirus (Ntaya serocomplex) and emerged in Europe, Spain, in 2010. The natural transmission cycle of this virus is perpetuated by Culex spp. mosquitoes and viraemic birds. The ability of BAGV to cause infection in several game birds from the family Phasianidae has been well-studied. BAGV is antigenically similar to other orthoflaviviruses from the Japanese encephalitis serocomplex, such as the West Nile and Usutu viruses, a circumstance which can lead to cross-reactivity in less specific serological techniques (e.g., ELISA). Severe implications in animal health has already been described, but some aspects of the dynamics of transmission and the limits of zoonotic potential of BAGV still need to be clarified. Further investigation focused on epidemiological surveillance in high-risk areas would be beneficial for prevention and control of new outbreaks. The present study is a systematic review of the BAGV reports in Europe.
- Histopathological findings and molecular diagnosis of Nocardia spp. in wild mammalsPublication . Matos, Manuela; João, S.; Matos, Ana; Figueira, Luís Manuel Faria; Cardoso, Luis; Coelho, Ana Cláudia; Pinto, Maria de LurdesNocardiosis is an emerging zoonosis caused by Nocardia spp. bacteria. The disease is linked to infections in immunocompromised individuals, being potentially lethal when it turns systemic. The development of reliable and valid diagnostic methods is crucial to the identification of these pathogenic agents, particularly in wild mammals, which are potential reservoirs in the wildlife. A convenience sample of 114 wild mammals belonging to the orders Canidae, Cervidae, Erinaceidae, Herpestidae, Mustelidae, Suidae, and Viverridae were studied.
- Molecular and serological screening support the lack of Coxiella burnetii circulation in wild birds of PortugalPublication . Loureiro, Filipa; Mesquita, João R.; Cardoso, Luis; Pintado, Cristina Santos; Gonçalves, S. Gomes; Matos, Ana; Soeiro, V.; Gonçalves, A.; Silva, Filipe; Matos, Manuela; Coelho, Ana CláudiaCoxiella burnetii is a highly infectious zoonotic pathogenic bacterium that has a major economic impact in the livestock industry throughout the world and causes unpredictable outbreaks in humans worldwide. Although it is known that birds are potential reservoirs of C. burnetii, their role in the epidemiological cycle of the pathogen has not been fully verified. Due to its non-specific symptoms and clinical signs, it is certainly an underdiagnosed disease. The objective of this study was to obtain more information on C. burnetii prevalence in wild birds in Portugal. Blood, plasma, and other tissue samples were obtained from wild birds admitted at wildlife rehabilitation centres in Portugal in the scope of passive surveillance. Antibodies specific to C. burnetii were screened using a commercial enzyme-linked immunosorbent assay according to the manufacturer’s instructions. Evidence of C. burnetii infection was sought based on the detection of bacterial DNA. No positive results were found, either in terms of antibodies to C. burnetii or molecular biology. These serological findings do not indicate the endemic circulation of C. burnetii in wild birds, which can be considered relevant information. However, a more complete and serialized approach over time is necessary to be able to make real inferences about the endemicity of the pathogen in the country and its dispersion among wild avian populations. qPCR results were also negative, a finding suggesting that this host population may not play a significant role in the transmission dynamics of C. burnetii. Given the importance of wild bird species as natural reservoirs of this zoonotic bacterium, we consider these data useful for multidisciplinary work in the prevention and control of Q fever, following a One Health approach.
- Occurrence of Coxiella burnetii in red deer (Cervus elaphus) in PortugalPublication . Pires, Humberto; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Santos Pintado, Cristina Maria Baptista; Figueira, Luís Manuel Faria; Mesquita, João R.; Matos, Ana; Coelho, Ana CláudiaOccurrence of Coxiella burnetii in red deer (Cervus elaphus) in Portugal.
- A preliminar study on Brucella spp. seroprevalence in wild boar (Sus scrofa) in PortugalPublication . Pires, Humberto; Matos, Manuela; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Pintado, Cristina; Santos Pintado, Cristina Maria Baptista; Figueira, Luis; Figueira, Luís Manuel Faria; Mesquita, João R.; Matos, Ana Cristina; Matos, Ana; Coelho, Ana CláudiaVarious species of the genus Brucella are highly virulent zoonotic agents. Brucella melitensis, B. abortus, and B. suis area broadly spread worldwide and animal brucellosis has a significant economic impact. This zoonotic disease affects both domestic pigs and wild pigs such as wild boar (Sus scrofa) In order to investigate the importance of wild boar (Sus scrofa) in the epidemiology of Brucella infection in Portugal, a serological survey was performed on samples from 184 wild boar fron the Centre region of Portugal.
- Screening wild birds for tick-borne zoonotic pathogens in PortugalPublication . Loureiro, F.; Mesquita, João R.; Cardoso, Luis; Santos-Silva, S.; Moreira, G.; Bento, J. T.; Soeiro, V.; Gonçalves, A.; Silva, F.; Barradas, P. F.; Matos, Ana Cristina; Matos, M.; Coelho, A. C.
- Serological evidence of Coxiella burnetii exposure in hunting dogs in Portugal.Publication . Pires, Humberto; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Santos Pintado, Cristina Maria Baptista; Figueira, Luís Manuel Faria; Mesquita, João R.; Matos, Ana; Coelho, Ana CláudiaCoxiella burnetii is a γ-Proteobacteria that is the etiologic agent of Q fever, a worldwide zoonosis. The role of dogs in the transmission of C. burnetii to humans is uncertain, and extensive seroprevalence studies of dogs have previously not been conducted in Portugal. A serologic survey was conducted for antibodies to C. burnetii in a sample of 90 hunting dogs from east-central Portugal, namely the municipalities of Proença-a-Nova and Castelo Branco, during the hunting season of 2021.
- Serological evidence of West Nile virus in wild birds in PortugalPublication . Loureiro, Filipa; Matos, Ana; Santos Pintado, Cristina Maria Baptista; Cardoso, Luis; Silva, Filiipe; Matos, Manuela; Coelho, Ana CláudiaWest Nile Virus (WNV) has a zoonotic transmission cycle. Avian species are considered the definitive hosts, and mosquitoes and other arthropods are the main vectors. Humans are considered accidental and dead-end hosts, since mammals are unable to efficiently amplify the virus. We aimed at studying serological prevalence of WNV circulation in wild birds in Portugal.
- Serological evidence of West Nile Virus infection in horses from the municipality of Castelo BrancoPublication . Loureiro, Filipa; Pires, Humberto; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Santos Pintado, Cristina Maria Baptista; Figueira, Luís Manuel Faria; Matos, Manuela; Coelho, Ana Cláudia; Matos, AnaWest Nile Virus (WNV) is an RNA virus from the family Flaviviridae. It is transmitted by several genera of mosquitoes, with Cullex spp. as the main vectors identified in Europe and North America. Birds are amplifying agents and important to the transmission cycle, during the viremia period. Equines are considered dead-ends hosts and are recognized as sentinels of the virus in many countries.