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Fontes, Maria da Conceição

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Now showing 1 - 8 of 8
  • Seropositivity of Coxiella burnetii in wild boar (Sus scrofa) and red deer (Cervus elaphus) in Portugal
    Publication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Mesquita, João Rodrigo; Matos, Ana Cristina; Coelho, Ana Cláudia
    Coxiella burnetii is a zoonotic microorganism that infects a wide range of wild and domestic species, causing the disease Q fever, frequently involving ticks as vectors. To better understand the occurrence of C. burnetii infection in wild boar (Sus scrofa) and red deer (Cervus elaphus), an epidemiological study was conducted in the Centre region of Portugal.
  • Prevalence and risk factors for hepatitis E virus in wild boar and red deer in Portugal
    Publication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Silva, Sérgio Santos; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Matos, Ana Cristina; Mesquita, João Rodrigo; Coelho, Ana Cláudia
    Hepatitis E virus (HEV) is a zoonotic foodborne virus with an annual infection prevalence of 20 million human cases, which seriously affects public health and economic development in both developed and developing countries. To better understand the epidemiology of HEV in Central Portugal, a cross-sectional study was conducted from 2016 to 2023 with sera samples from wild ungulates. The seroprevalence and risk factors for HEV seropositivity were evaluated in the present study. Specifically, antibodies against HEV were determined by a commercial enzyme-linked immunesorbent assay (ELISA). Our results show that in the 650 sera samples collected from 298 wild red deer and 352 wild boars in Portugal, 9.1% red deer and 1.7% wild boar were positive for antibodies to HEV. Regarding age, the seropositivity in juvenile wild ungulates was 1.3%, whereas it was 7.2% in adults. Logistic regression models investigated risk factors for seropositivity. The odds of being seropositive was 3.6 times higher in adults than in juveniles, and the risk was 4.2 times higher in red deer than in wild boar. Both wild ungulate species were exposed to HEV. The higher seroprevalence in red deer suggests that this species may make a major contribution to the ecology of HEV in Central Portugal. Further research is needed to understand how wildlife affects the epidemiology of HEV infections in Portugal.
  • Seropositivity for Coxiella burnetii in wild boar (Sus scrofa) and red deer (Cervus elaphus) in Portugal
    Publication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Mesquita, João Rodrigo; Matos, Ana Cristina; Coelho, Ana Cláudia
    Q fever is caused by the pathogen Coxiella burnetii and is a zoonosis that naturally infects goats, sheep, and cats, but can also infect humans, birds, reptiles, or arthropods. A survey was conducted for the detection of antibodies against C. burnetii in a sample of 617 free-ranging wild ruminants, 358 wild boar (Sus scrofa) and 259 red deer (Cervus elaphus), in east–central Portugal during the 2016–2022 hunting seasons. Only adult animals were sampled in this study. Antibodies specific to C. burnetii were detected using a commercial enzyme-linked immunosorbent assay (ELISA; IDVet®, Montpellier, France) according to the manufacturer’s instructions. The seroprevalence of C. burnetii infection was 1.5% (n = 9; 95% confidence interval [CI]: 0.7–2.8%). Antibodies against C. burnetii were detected in 4/358 wild boar (1.1%; 95% CI: CI: 0.3–2.8%) and 5/259 red deer (1.9%; 0.6–4.5%). Results of the present study indicate that antibodies against C. burnetii were present in wild boar and red deer in Portugal. These findings can help local health authorities to focus on the problem of C. burnetii in wildlife and facilitate the application of a One Health approach to its prevention and control.
  • High seroprevalence of Brucella spp. in wild boar and red deer in the Centre of Portugal
    Publication . Pires, Humberto; Matos, Manuela; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Pintado, Cristina Santos; Figueira, Luís; Matos, Ana Cristina; Mesquita, João Rodrigo; Coelho, Ana Cláudia
    High seroprevalence of Brucella spp. in wild boar and red deer in the Centre of Portugal.
  • Occurrence of Coxiella burnetii in red deer in Portugal
    Publication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Mesquita, João Rodrigo; Matos, Ana Cristina; Coelho, Ana Cláudia
    Occurrence of Coxiella burnetii in red deer in Portugal.
  • Seroepidemiological study of Hepatitis E virus (Hev) infection in large game animals in the Centre of Portugal
    Publication . Pires, Humberto; Matos, Manuela; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Pintado, Cristina Santos; Figueira, Luís; Matos, Ana Cristina; Mesquita, João Rodrigo; Coelho, Ana Cláudia
    Seroepidemiological study of Hepatitis E virus (Hev) infection in large game animals in the Centre of Portugal.
  • Knowledge about COVID-19: best practices in the North of Portugal and the importance of health education in the prevention of pandemic events
    Publication . Oliveira, Joana; Rodrigues, Isilda; Matos, Manuela; Carvalho, Isabel; Matos, Ana Cristina; Figueira, Luís; Fontes, Maria da Conceição; Alegria, Nuno; Lopes, Ana Patrícia; Vale, Beatriz do; Cardoso, Luís; Coelho, Ana Cláudia
    The rapid global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made COVID-19 one of the biggest pandemics of all time, with several devastating public health challenges. In this study, we investigated the knowledge towards COVID-19 best practices in the north of Portugal. Methods: A descriptive cross-sectional study was performed with a convenience sample of the population of northern Portugal to assess their knowledge about COVID-19, SARS-CoV-2 and measures to prevent and mitigate pandemics. An online validated questionnaire was completed by 411 participants, from September to October 2020. Results: The overall correct knowledge was 81.3%, which indicates a good knowledge by the northern Portuguese respondents about COVID-19. The correct answer score differed considerably between men and women, being significantly higher among the latter (12.28 ± 1.22; p = 0.011). Moreover, the highest knowledge was observed in participants who attended high school or above (12.27 ± 1.21; p < 0.000). Conclusion: This study contributes to the analysis of COVID-19 knowledge by the northern Portuguese population, emphasizes the crucial role of health education in the control and mitigation of the COVID-19 pandemic, and provides field-based evidence to prevent the next pandemic event.
  • Hunting for answers: Assessing Brucella spp. seroprevalence and risks in red deer and wild boar in Central Portugal
    Publication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Silva, Sérgio Santos; Matos, Manuela; Pintado, Cristina Santos; Roque, Natália; Fonseca, Leonardo Filipe; Morgado, Inês; Dias, Ana Sofia; Figueira, Luis; Matos, Ana Cristina; Mesquita, João Rodrigo; Coelho, Ana Cláudia
    Between 2016 and 2023, a cross-sectional study was conducted in the central region of Portugal in order to better understand the epidemiology and public health risks resulting from the handling and consumption of game animals infected with Brucella spp. The seroprevalence and risk factors for Brucella spp. seropositivity were evaluated. Antibodies against Brucella spp. were determined using a commercial enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions. Results showed that in the 650 serum samples collected from red deer (n = 298) and wild boars (n = 352) in Portugal, 21.7% (n = 141; 95% CI: 18.6–25.1%) tested positive. Wild boar had a significantly higher prevalence (35.5%; 95% CI: 30.5–40.8%) than red deer (5.4%, 95% CI: 3.1–8.6%; p ≤ 0.001). Risk factors for seropositivity were investigated using multivariable logistic regression models. The odds of being seropositive was 8.39 (95% CI: 4.75–14.84; p ≤ 0.001) times higher in wild boar than in red deer. Correlations between sex, age, body condition, and seropositivity could not be observed. The higher seroprevalence in wild boar suggests that this species may primarily contribute to the Brucella spp. ecology in central Portugal