Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of an outbreak by carbapenem-resistant Klebsiella pneumoniae

Silva, Liliana; Rodrigues, Carla; Lira, Agostinho; Leão, Mariana; Mota, Margarida; Lopes, Paulo; Novais, Ângela; Peixe, Luísa

http://hdl.handle.net/10400.11/7369

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Authors

Silva, Liliana

Abstract(s)

The spread of multidrug-resistant (MDR) Klebsiella pneumoniae in the nosocomial setting represents a big challenge to infection control teams. We have recently developed a simple spectroscopic-based method with excellent accuracy, turnaround time and cost-effectiveness (Rodrigues et al. mSystems 2020) for bacterial typing. Here, we applied our method in a real clinical context to support early identification of an outbreak involving KPC-3-producing K. pneumoniae ST147 isolates. Our results further support that attenuated total reflectance Fourier transform infrared (FT-IR) spectroscopy can provide enough information to support early and adequate infection control measures and therapeutic choices in the context of nosocomial outbreaks and hospital surveillance.

Keywords

Bacterial typing Nosocomial infection Carbapenemases KPC-3 Capsule type Capsular polysaccharides

URI

http://hdl.handle.net/10400.11/7369

Citation

SILVA, L. [et al.] (2020) - Fourier transform infrared (FT-IR) spectroscopy typing: a real-time analysis of an outbreak by carbapenem-resistant Klebsiella pneumoniae. European Journal of Clinical Microbiology & Infectious Diseases. ISSN 0934-9723. Vol. 39, p.2471–2475 . DOI: 0.1007/s10096-020-03956-y