Browsing by Author "Pires, Humberto"
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- Análise geoespacial aplicada ao rastreio sero-epidemiológico da leishmaniose canina na região centro de PortugalPublication . Pires, Humberto; Cortes, Helder; Nunes, Telmo; Monteiro, Fernando; Cardoso, Luís; Matos, Ana Cristina; Martins, Manuel
- Brucella spp. seroprevalence in wild ungulates in PortugalPublication . Pires, Humberto; Matos, Manuela; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Santos Pintado, Cristina Maria Baptista; Figueira, Luís Manuel Faria; Mesquita, João R.; Matos, Ana; Coelho, Ana CláudiaBrucella spp. seroprevalence in wild ungulates in Portugal.
- Evidence for sporadic Coxiella burnetii cxcretion in sheep and goat milk, Central Portugal.Publication . Pires, Humberto; Pereira, Maria A.; Nóbrega, Carmen; Cruz, Rita; Esteves, Fernando; Vala, Helena; Silva, Sérgio Santos; Barradas, Patrícia; Matos, Ana Cristina; Coelho, Ana Cláudia; Mesquita, João RodrigoEvidence for sporadic Coxiella burnetii cxcretion in sheep and goat milk, Central Portugal.
- Evidence for sporadic Coxiella burnetii excretion in sheep milk, central PortugalPublication . Pires, Humberto; Silva, S. Santos; Cruz, A.; Cardoso, Luís; Lopes, A.P.; Pereira, M.A.; Nóbrega, C.; Mega, A.C.; Santos, C.; Cruz, R.; Esteves, F.; Vala, H.; Matos, Ana; Barradas, P.F.; Coelho, A.C.; Mesquita, J.R.Coxiella burnetii, the causative agent of Q fever, is a globally distributed zoonosis, with cattle, sheep, and goats considered the main reservoirs of the disease (Agger et al,., 2013; Bento et al., 2023). Human transmission primarily occurs through inhaling infections aerosols from milk, faeces, urina, and birth products from infected ruminants. This study used a 2-year longitudinal approach to investigate C. burnetii excretion in bulk tank milk samp+les from sheep in central Portugal.
- Geospatial analysis applied to seroepidemiological survey of canine leishmaniosis in east-central PortugalPublication . Pires, Humberto; Martins, Manuel; Matos, Ana Cristina; Cardoso, Luís; Monteiro, Fernando; Roque, Natália; Nunes, Telmo; Gottstein, Bruno; Cortes, HelderDespite the high prevalence of canine Leishmania infantum infection in Portugal, significant differences associated with different risk factors can be found between geographically contiguous areas. In this study, a geographical area within the central region of Portugal (municipalities of Proença-a-Nova, Mação and Vila de Rei) was investigated. An epidemiological survey involved the analysis by an indirect enzyme-linked immunosorbent assay of serum samples collected during the anti-rabies vaccination campaign from 282 dogs. Geospatial analysis showed the distribution of geospatial prevalence of leishmaniosis and has delimited two areas (clusters) with a statistically significant higher risk of seropositivity in dogs (p = 0.003 and p = 0.027, for clusters 1 and 2, respectively). The highest seroprevalence (56.0%; CI: 41.2-70.0) was found in Vila de Rei. Five land occupation types showed a possible influence on the geographic distribution of seropositivity, with statistically significant differences between seropositive and seronegative dogs. Land occupied by temporary irrigated crops (p = 0.026), olive groves (p = 0.013), complex cultural systems and parcelling (p = 0.021), open forests, logging and new plantations (p = 0.043) and watercourses (p = 0.012) influenced the geographical distribution of canine Leishmania infection. Seropositive dogs had a greater average area of occupied land (i.e. open forests, logging and new plantations) than the seronegative ones (3.1439 km2 versus 2.5650 km2, respectively; p = 0.043).
- High seroprevalence of Brucella spp. in wild boar and red deer in the Centre of PortugalPublication . Pires, Humberto; Matos, Manuela; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Pintado, Cristina Santos; Figueira, Luís; Matos, Ana Cristina; Mesquita, João Rodrigo; Coelho, Ana CláudiaHigh seroprevalence of Brucella spp. in wild boar and red deer in the Centre of Portugal.
- Hunting for answers: Assessing Brucella spp. seroprevalence and risks in red deer and wild boar in Central PortugalPublication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Silva, Sérgio Santos; Matos, Manuela; Pintado, Cristina Santos; Roque, Natália; Fonseca, Leonardo Filipe; Morgado, Inês; Dias, Ana Sofia; Figueira, Luis; Matos, Ana Cristina; Mesquita, João Rodrigo; Coelho, Ana CláudiaBetween 2016 and 2023, a cross-sectional study was conducted in the central region of Portugal in order to better understand the epidemiology and public health risks resulting from the handling and consumption of game animals infected with Brucella spp. The seroprevalence and risk factors for Brucella spp. seropositivity were evaluated. Antibodies against Brucella spp. were determined using a commercial enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions. Results showed that in the 650 serum samples collected from red deer (n = 298) and wild boars (n = 352) in Portugal, 21.7% (n = 141; 95% CI: 18.6–25.1%) tested positive. Wild boar had a significantly higher prevalence (35.5%; 95% CI: 30.5–40.8%) than red deer (5.4%, 95% CI: 3.1–8.6%; p ≤ 0.001). Risk factors for seropositivity were investigated using multivariable logistic regression models. The odds of being seropositive was 8.39 (95% CI: 4.75–14.84; p ≤ 0.001) times higher in wild boar than in red deer. Correlations between sex, age, body condition, and seropositivity could not be observed. The higher seroprevalence in wild boar suggests that this species may primarily contribute to the Brucella spp. ecology in central Portugal
- Laboratório de ParasitologiaPublication . Matos, Ana Cristina; Brida, Telma; Figueira, Luís; Brancal, Hugo; Pires, Humberto; Gavinhos, Catarina; Mateus, Paulo; Martins, ManuelLaboratório de Parasitologia.
- Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, central PortugalPublication . Pires, Humberto; Silva, Sérgio Santos; Cruz, Andreia V.S.; Cardoso, Luís; Lopes, Ana Patrícia; Pereira, Maria A.; Nóbrega, Carmen; Mega, Ana Cristina; Santos, Carla; Cruz, Rita; Esteves, Fernando; Vala, Helena; Matos, Ana Cristina; Barradas, Patrícia F.; Coelho, Ana Cláudia; Mesquita, João RodrigoCoxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk tank milk samples of sheep from a mountain plateau in central Portugal, with sampling conducted during the years 2015 and 2016. From a total of 156 bulk tank milk samples tested by qPCR, only one showed to be positive for C. burnetii (1.28% [95%CI: 0.03–6.94]), from 2015, the first year of collection. Bidirectional sequencing and phylogenetic analysis of IS1111 transposase partial region confirmed the presence of C. burnetii DNA. The presence of C. burnetii in raw milk samples highlights the necessity for additional research to determine if raw milk is a potential source for human infection. Animal health surveillance and prevention measures against this zoonotic disease should be considered.
- Occurrence of Coxiella burnetii in red deer in PortugalPublication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Mesquita, João Rodrigo; Matos, Ana Cristina; Coelho, Ana CláudiaOccurrence of Coxiella burnetii in red deer in Portugal.
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