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- Géneros parasitários mais frequentes em pequenos ruminantes na Beira Interior Sul (Portugal).Publication . Matos, Ana Cristina; Brida, Telma; Gavinhos, Catarina; Cardoso, Pedro; Figueira, Luís; Martins, Manuel
- TSE monitoring in wildlife epidemiology, transmission, diagnosis, genetics and controlPublication . Machado, Carla; Orge, Leonor; Pires, Isabel; Gama, Adelina; Esteves, Alexandra; Mendonça, Paula; Matos, Ana Cristina; Alves, Anabela; Lima, Carla; Bastos, Estela; Seixas, Fernanda; Silva, Filipe; Silva, João; Figueira, Luís; Pinto, Madalena Vieira; Pinto, Maria de Lurdes; Gonçalves-Anjo, Nuno; Tavares, Paula; Carvalho, Paulo; Sargo, Roberto; Pires, Maria dos AnjosAmong the transmissible spongiform encephalopathies (TSEs), chronic wasting disease (CWD) in cervids is now the rising concern within Europe. CWD will be outlined in this chapter gathering its epidemiology, transmission, diagnosis, genetics, and control. Prion diseases are fatal neurodegenerative diseases characterized by the accumulation of an abnormal isoform of the prion protein (PrPc), usually designated by PrPsc or prion. CWD is a prion disease of natural transmission affecting cervids detected mainly in North America. The first European case was detected in Norway, in 2016, in a wild reindeer; until April 2018, a total of 23 cases were described. The definite diagnosis is postmortem, performed in target areas of the brain and lymph nodes. Samples are first screened using a rapid test and, if positive, confirmed by immunohistochemistry and Western immunoblotting. It is not possible to establish a culling plan based on the genotype, once affected animals appear with all genotypes. However, some polymorphisms seem to result in longer incubation periods or confer a reduced risk. The control is not easy in captive cervids and even more in the wildlife; some recommendations have been proposed in order to understand the danger and impact of CWD on animal and public health.
- Seropositivity of Coxiella burnetii in wild boar (Sus scrofa) and red deer (Cervus elaphus) in PortugalPublication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Mesquita, João Rodrigo; Matos, Ana Cristina; Coelho, Ana CláudiaCoxiella burnetii is a zoonotic microorganism that infects a wide range of wild and domestic species, causing the disease Q fever, frequently involving ticks as vectors. To better understand the occurrence of C. burnetii infection in wild boar (Sus scrofa) and red deer (Cervus elaphus), an epidemiological study was conducted in the Centre region of Portugal.
- Tuberculose disseminada com manifestações cerebrais, pulmonares, ganglionares e intestinais em raposasPublication . Matos, Ana Cristina; Coelho, Ana Cláudia; Figueira, Luís; Morais, Márcia; Dias, Ana Paula; Martins, Maria Helena; Martins, Manuel; Pinto, Maria de Lurdes; Matos, ManuelaTuberculose disseminada com manifestações cerebrais, pulmonares, ganglionares e intestinais em raposas.
- Estudo do parasitismo animal na região da Beira Baixa. Resultados preliminaresPublication . Martins, Manuel; Brida, Telma; Gavinhos, Catarina; Matos, Ana CristinaEstudo do parasitismo animal na região da Beira Baixa. Resultados preliminares.
- Survey of Mycobacterium avium subspecies paratuberculosis in road-killed wild carnivores in PortugalPublication . Matos, Ana Cristina; Figueira, Luís; Martins, Maria Helena; Loureiro, Filipa; Pinto, Maria de Lurdes; Matos, Manuela; Coelho, Ana CláudiaA survey to determine the occurrence of Mycobacterium avium subsp. paratuberculosis (MAP) in wild carnivores in Portugal was conducted by testing samples from road-killed animals between 2009 and 2012. Postmortem examinations were performed and tissues were collected from wild carnivores representing four families and six different species, with a total of 74 animals analyzed. Cultures were performed by using Löwenstein-Jensen and Middlebrook 7H11 solid media and acid-fast isolates were identified by polymerase chain reaction (PCR) and mycobactin dependency characteristics. Tissues were also screened for MAP by directly extracting DNA and testing for the MAP-specific sequences. The occurrence of infected animals (an animal had at least one tissue that was positive for culture or direct PCR) was 27.0% (n = 20). MAP was isolated from culture of 25 tissue samples (3.8%) and was detected by direct PCR in 40 (6.0%) samples. Infection was recorded in 5/6 studied species: 7/49 (14.3%) red foxes (Vulpes vulpes), 3/3 (100%) beech martens (Martes foina), 2/4 (50.0%) Eurasian otters (Lutra lutra), 7/15 (46.7%) Egyptian mongooses (Herpestes ichneumon), and 1/1 (100%) European badger (Meles meles). These species represent three different taxonomic families: Canidae (14.3% were positive), Mustelidae (75.0% were positive), and Herpestidae (46.7% were positive). The results of this study confirm the presence of MAP infection in wild carnivores in Portugal.
- Pulmonary lesions consistent with disseminated adiaspiromycosis in Egyptian mongooses (Herpestes ichneumon) from PortugalPublication . Matos, Ana Cristina; Figueira, Luís; Martins, Maria Helena; Matos, Manuela; Pires, Maria dos Anjos; Coelho, Ana Cláudia; Pinto, Maria de LurdesPulmonary lesions consistent with disseminated adiaspiromycosis in Egyptian mongooses (Herpestes ichneumon) from Portugal.
- Five years in the detection of Talaromyces marneffei in Portugal. It’s time to think about!Publication . Coelho, Ana Cláudia; Matos, Ana Cristina; Figueira, Luís; Soares, Ana Sofia; Ferreira, R.; Ferreira, T.; Santos, Marlene; Matos, Manuela
- Inspecção sanitária : tipificação das rejeições totais em pequenos ruminantesPublication . Matos, Ana Cristina; Menezes, A.M.; Reis, M.O. Portela dos; Domingues, H.; Figueira, Luís; Brida, Telma; Martins, ManuelCom base no conceito de inspecção integrada que se efectua desde a produção ao consumo e verificando a percentagem de pequenos ruminantes rejeitados no matadouro da Oviger S.A. – Alcains, surgiu a necessidade de identificar e quantificar as rejeições totais, permitindo assim ao corpo de Inspecção Sanitária redireccionar as prioridades da inspecção para áreas mais convenientes e estabelecer inter-relações com as organizações de produtores pecuários (OPP’s) onde estão integradas as explorações de origem dos animais rejeitados, inter-relações que são de extrema importância no controlo de zoonoses e na defesa da saúde pública e animal. Para a realização deste trabalho foram utilizados dados correspondentes ao abate de pequenos ruminantes, durante o período de Janeiro de 2007 a Dezembro de 2009. Foram determinados os índices de rejeição total por ano e identificaram-se as diversas alterações e patologias que motivaram a sua rejeição. Em relação ao número total de pequenos ruminantes abatidos, observou-se uma diminuição do nº total de cabeças, tendo o índice de rejeição total também diminuído de 1,32% para 0,96% em ovinos e de 0,8 para 0,72% em caprinos. A principal causa de rejeição deveu-se ao abate sanitário (Brucelose; TSE’s), que durante o período considerado representaram para os ovinos 42,27% do total das rejeições e para os caprinos 16,4%. Estas rejeições correspondem a avultados prejuízos económicos mas permitem ao consumidor uma maior segurança alimentar. Não considerando as rejeições provenientes dos abates sanitários, as principais causas de rejeição total de ovinos e caprinos foram as pneumonias (36,34%/24,24%) e hidroémia/hidrocelo/caquexia (15,77%/20,13%).
- Chronic wasting disease risk assessment in Portugal - Genetic variability preliminary results and future perspectivesPublication . Pereira, Jorge C.; Gonçalves-Anjo, Nuno; Bastos, Estela; Rocha, Sara; Figueira, Luís; Matos, Ana Cristina; Machado, Carla; Silva, João; Mendonça, Paula; Carvalho, Paulo; Tavares, Paula; Lima, Carla; Alves, Anabela; Esteves, Alexandra; Pinto, Maria de Lurdes; Pires, Isabel; Gama, Adelina; Sargo, Roberto; Silva, Filipe; Seixas, Fernanda; Pinto, Madalena Vieira; Orge, Leonor; Pires, Maria dos Anjos