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- Olivicultura sustentável e qualidade do Azeite "Galega" na Beira BaixaPublication . Peres, Maria de Fátima; Ferreira, Armando; Pintado, Cristina Santos; Henriques, Luís; Coutinho, J.P.; Luz, J.P.; Vitorino, Conceição; Gouveia, Cecília; Silva, M.C.A.; Outão, Fernanda; Fragoso, Preciosa; Peres, Cidália; Antunes, Vítor; Domingos, Ana; Pereira, JoãoO presente trabalho avalia a qualidade do azeite ‘Galega’ proveniente de olivais em produção integrada e em modo de produção biológico situados na Beira Baixa. São apresentados os resultados de diversos parâmetros físico-químicos de azeites virgens, nas campanhas 2004/05 e 2005/06, em duas datas de colheita. A caracterização química e sensorial do azeite ‘Galega’ revela a importância da data de colheita na sua qualidade, sobretudo nos olivais em modo de produção biológico. Todos os azeites estudados mostraram elevada estabilidade oxidativa.
- Seropositivity of Coxiella burnetii in wild boar (Sus scrofa) and red deer (Cervus elaphus) in PortugalPublication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Mesquita, João Rodrigo; Matos, Ana Cristina; Coelho, Ana CláudiaCoxiella burnetii is a zoonotic microorganism that infects a wide range of wild and domestic species, causing the disease Q fever, frequently involving ticks as vectors. To better understand the occurrence of C. burnetii infection in wild boar (Sus scrofa) and red deer (Cervus elaphus), an epidemiological study was conducted in the Centre region of Portugal.
- Prevalence and risk factors for hepatitis E virus in wild boar and red deer in PortugalPublication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Silva, Sérgio Santos; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Matos, Ana Cristina; Mesquita, João Rodrigo; Coelho, Ana CláudiaHepatitis E virus (HEV) is a zoonotic foodborne virus with an annual infection prevalence of 20 million human cases, which seriously affects public health and economic development in both developed and developing countries. To better understand the epidemiology of HEV in Central Portugal, a cross-sectional study was conducted from 2016 to 2023 with sera samples from wild ungulates. The seroprevalence and risk factors for HEV seropositivity were evaluated in the present study. Specifically, antibodies against HEV were determined by a commercial enzyme-linked immunesorbent assay (ELISA). Our results show that in the 650 sera samples collected from 298 wild red deer and 352 wild boars in Portugal, 9.1% red deer and 1.7% wild boar were positive for antibodies to HEV. Regarding age, the seropositivity in juvenile wild ungulates was 1.3%, whereas it was 7.2% in adults. Logistic regression models investigated risk factors for seropositivity. The odds of being seropositive was 3.6 times higher in adults than in juveniles, and the risk was 4.2 times higher in red deer than in wild boar. Both wild ungulate species were exposed to HEV. The higher seroprevalence in red deer suggests that this species may make a major contribution to the ecology of HEV in Central Portugal. Further research is needed to understand how wildlife affects the epidemiology of HEV infections in Portugal.
- Seropositivity for Coxiella burnetii in wild boar (Sus scrofa) and red deer (Cervus elaphus) in PortugalPublication . Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina Santos; Figueira, Luís; Mesquita, João Rodrigo; Matos, Ana Cristina; Coelho, Ana CláudiaQ fever is caused by the pathogen Coxiella burnetii and is a zoonosis that naturally infects goats, sheep, and cats, but can also infect humans, birds, reptiles, or arthropods. A survey was conducted for the detection of antibodies against C. burnetii in a sample of 617 free-ranging wild ruminants, 358 wild boar (Sus scrofa) and 259 red deer (Cervus elaphus), in east–central Portugal during the 2016–2022 hunting seasons. Only adult animals were sampled in this study. Antibodies specific to C. burnetii were detected using a commercial enzyme-linked immunosorbent assay (ELISA; IDVet®, Montpellier, France) according to the manufacturer’s instructions. The seroprevalence of C. burnetii infection was 1.5% (n = 9; 95% confidence interval [CI]: 0.7–2.8%). Antibodies against C. burnetii were detected in 4/358 wild boar (1.1%; 95% CI: CI: 0.3–2.8%) and 5/259 red deer (1.9%; 0.6–4.5%). Results of the present study indicate that antibodies against C. burnetii were present in wild boar and red deer in Portugal. These findings can help local health authorities to focus on the problem of C. burnetii in wildlife and facilitate the application of a One Health approach to its prevention and control.
- Essential oils of two portuguese endemic species of lavandula as a source of antifungal and antibacterial agentsPublication . Domingues, Joana Lopes; Goulão, Manuela; Delgado, F.M.G.; Gonçalves, José Carlos; Gonçalves, Joana; Pintado, Cristina SantosChemical investigations of L. stoechas subsp. luisieri and L. pedunculata essential oils were analyzed by GC-MS, and the antimicrobial activity was performed against bacteria and fungi isolated from food sources. The cytotoxicity of the essential oil was performed in NHDF cells using the MTT method. According to the results, the main compounds of L. stoechas subsp. luisieri essential oil were trans-α-necrodyl acetate (40.2%), lavandulyl acetate (11%), and trans-α-necrodol (10.4%), while fenchone (50.5%) and camphor (30.0%) in L. pedunculata essential oil. The antifungal activity of essential oils was confirmed with MIC values ranging from 1.2 to 18.7 µL/mL; for bacteria, it ranged from 4.7 to 149.3 µL/mL. Both the Lavandula species tested showed low or equal MIC and MBC/MFC values for L. stoechas subsp. luisieri essential oil, revealing greater efficacy in antimicrobial activity. The L. stoechas subsp. luisieri essential oil revealed cytotoxic effects (30 ± 2% of cell viability) in NHDF cells at all concentrations tested.
- Essential oils of Lavandula stoechas subsp. luisieri as antifungal agent against fungi from strawberry tree fruitPublication . Domingues, Joana Lopes; Delgado, F.M.G.; Gonçalves, José Carlos; Pintado, Cristina SantosEssential oils of Lavandula stoechas subsp. luisieri as antifungal agent against fungi from strawberry tree fruit
- Implementação da técnica AFLP (Amplified Fragment Length Polymorphisms) para a tipagem molecular de Listeria spp.Publication . Leitão, Ana Maria; Reis, C.M.G.; Pedroso, Ana Sofia; Dinis, Patrícia; Pintado, Cristina SantosCom este trabalho foi possível implementar na ESACB a técnica de biologia molecular Amplified Fragment Length Polymorphisms (AFLP), com vista à tipagem molecular de culturas de Listeria monocytogenes e outras Listeria spp., as quais tinham sido isoladas maioritariamente de queijo, leite cru e ambiente de uma queijaria. A técnica apresentou uma boa reprodutibilidade e poder discriminatório para Listeria monocytogenes e L. innocua. Relativamente à espécie L. ivanovii, obtiveram-se perfis com um reduzido número de bandas. Verificou-se a presença de uma banda com aproximadamente 850 pb, comum às amostras estudadas de L. monocytogenes e L. innocua.
- Soluções sustentáveis para a valorização de produtos naturais e resíduos industriais de origem vegetalPublication . Henriques, Marta; Silva, Aida Moreira da; Veloso, Ana; Galhano, Cristina; Pintado, Cristina Santos; Delgado, F.M.G.; Ferreira, Fernanda; Seabra, Inês; Rodrigues, Ivo; Noronha, João; Castro, Luís; Paulo, Luísa; Goulão, Manuela; Barroca, Maria João; Moreira, Maria João; Pinheiro, Nazaré; Santos, Sandra; Dias, SusanaSOS Valor caracteriza extratos de folhas e flores de medronheiro (Arbutus unedo) e testa a sua capacidade de combater doenças neurológicas e efeito nematicida
- Control of pathogenic and spoilage microorganisms from cheese surface by whey protein films containing malic acid, nisin and natamycinPublication . Pintado, Cristina Santos; Ferreira, Maria A.S.S.; Sousa, IsabelThe inhibitory effects of nisin, natamycin and malic acid, incorporated in whey protein films with pH 3, were investigated alone or with addition of sucrose esters, Tween80 or EDTA. Water vapour permeability measurements and mechanical and rheological tests were also assessed. EDTA and Tween80 did not significantly (P < 0.05) influence the inhibitory activity of films against Pseudomonas aeruginosa and Yarrowia lipolytica in contrast with the improved effect against Listeria monocytogenes, Penicillium commune and Penicillium chrysogenum. Sucrose esters reduced significantly (P < 0.05) the inhibitory effect for Y. lipolytica and Penicillium spp. The present work provides an antimicrobial film formulation with potential to be a hurdle against spoilage and pathogenic microorganisms isolated from cheese surface.
- High seroprevalence of Brucella spp. in wild boar and red deer in the Centre of PortugalPublication . Pires, Humberto; Matos, Manuela; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Pintado, Cristina Santos; Figueira, Luís; Matos, Ana Cristina; Mesquita, João Rodrigo; Coelho, Ana CláudiaHigh seroprevalence of Brucella spp. in wild boar and red deer in the Centre of Portugal.