CATAA - Centro de Apoio Tecnológico Agro-Alimentar
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- Análise química de mel para garantir conformidade de produtoPublication . Resende, Mafalda; Paulo, Luísa; Nunes, André; Antunes, Paulo
- Antioxidant and dual dose-dependent antigenotoxic and genotoxic properties of an ethanol extract of propolisPublication . Cruz, Maria Teresa; Antunes, Paulo; Paulo, Luísa; Ferreira, A.M.; Cunha, A.; Aguiar, C. Almeida; Oliveira, RuiPropolis is a resinous product made by honeybees from plant-derived materials, with high content ofpolyphenols associated to several beneficial bioactivities with potential use as a natural food additive forpreservation and as a functional food ingredient. A Portuguese propolis ethanol extract (C.EE) protectedSaccharomyces cerevisiaecells from loss of viability upon exposure to H2O2, both in co- and in pre-incubation experiments. Results obtained with the comet assay suggest that lower concentrations areantigenotoxic while at higher concentrations a genotoxic effect prevails, which correlates with thecytotoxicity of high concentrations of C.EE. Flow cytometry analysis with dichlorofluorescein indicatesthat C.EE induced intracellular antioxidant activityin vivo. Overall the results suggest that C.EE isantigenotoxic but is also toxic at higher concentrations. This dual effect could be explained by thepresence of compounds known to interfere with DNA synthesis and/or cell proliferation, such as caffeicacid phenethyl ester (CAPE) and chrysin, together with antioxidants, like kaempferol, pinobanksin andpinocembrin.
- Caracterização da composição em açúcares do mel da região de Castelo BrancoPublication . Antunes, Paulo; Resende, Mafalda; Anjos, O.Caracterização da composição em açúcares do mel da região de Castelo Branco.
- Chemical composition of largemouth bass (Micropterus salmoides) filletPublication . Rodrigues, A.M.; Antunes, Paulo; Andrade, L.P.Largemouth bass (Micropterus salmoides) is a freshwater fish originating from the United States of America (USA). This specie was introduced in Portugal (Azores) in the end of the XIX Century. Like in the USA, largemouth bass is one of the most popular freshwater sports fish in Portugal and it is very important in regional cuisine, especially in the countryside. However, there’s a lack of information about the chemical composition of largemouth bass fillet. All eaten largemouth bass in Portugal are caught in large dams and small irrigation dams because there’s no largemouth bass aquaculture industry. The aim of this work was to evaluate the chemical composition of largemouth bass fillet collected in an irrigation reservoir (39º49’27.89’’ N; 07º26’57.92’’ W) located in the Central region of Portugal. Thirteen largemouth bass were collected (average weight 349.85g ±74.23; average length 27.22cm ±1.43; average K condition factor 1.71 ±0.14) and were frozen during seven days. The cutting carcasses and the filet chemical analyses (moisture, protein, fat and ash) took place in the laboratory. In the carcass, the average amounts of viscera, spine, head, skin and filet were, respectively, 8.52% (±1.41), 17.24% (±1.29), 23.06% (±1.85), 7.58% (±0.60) and 43.59% (±1.91). In the filet, the average amounts of moisture, protein, fat and ash were, respectively, 77.67% (±1.07), 18.46% (±0.83), 0.90% (±0.31) and 1.20% (±0.05). We concluded that largemouth bass has a good filet yield with very low fat and high level of protein contents. In fact, as far as we know, these are the first results of carcass and filet characterization of largemouth bass collected in Portugal.
- Composição em proteína, gordura e ácidos gordos de filetes de achigãs (Micropterus salmoides Lacépède, 1802) submetidos a três regimes alimentares diferentesPublication . Andrade, L.P.; Santos, C.; Resende, Mafalda; Antunes, Paulo; Rodrigues, A.M.Composição em proteína, gordura e ácidos gordos de filetes de achigãs (Micropterus salmoides Lacépède, 1802) submetidos a três regimes alimentares diferentes.
- Composição nutricional do filete de três espécies piscícolas de águas interiores com interesse gastronómicoPublication . Oliveira, Vítor Hugo; Antunes, Paulo; Resende, Mafalda; Andrade, L.P.; Rodrigues, A.M.Composição nutricional do filete de três espécies piscícolas de águas interiores com interesse gastronómico.
- Convective dehydration processing of peachPublication . Resende, Mafalda; Paulo, Luísa; Nunes, André; Silveira, Ana; Pintado, Cristina Miguel; Antunes, PauloDehydration of peach is an alternative to enhance storage stability, mínimize packaging requirement and reducing post-harvest cost without a sensorial and nutritional quality loss. Preservation of fruits through sun drying techniques is practiced in Cova da Beira (a Portuguese region that has a protected geographical indication for peach), as a homemade process without quality and safety control. The aim of this work was to prepare quality dehydrated products based on a convective drying process with low cost. This process has short drying time and controlled temperature that causes minimal damage to the product without added preservatives or sugar. The samples of ‘Baby Gold’ cultivar (Prunus persica) obtained from local producer were peeled, laminated and immediately dehydrated. Samples were stored in sealed plastic containers at 25°C for subsequent analysis. In the drying process were controlled forced convection air velocity of 1.25 to 1.50 m/s, air temperature between 45 and 75°C and drying time between 7 to 11 hours. During dehydration process temperature and fruit moisture were controlled. pH, acidity, soluble solids content (SSC) and mesophilic aerobic count were performed in fresh and dehydrated fruit. Dehydrated peach were similar to homemade products. Moisture was below 12%, without significant variation of SSC and acidity (on a dry basis). No deleterious effect occurred on peach mesophilic aerobic count, since a slight decrease was registered between fresh and dehydrated fruit.
- High total phenol content, antioxidant activity and mineral content in ‘Sweetheart’ cherry peelPublication . Resende, Mafalda; Paulo, Luísa; Antunes, Paulo; Pintado, Cristina MiguelCherry is used for several processed products, resulting in a large volume of subproducts, mainly stones and peel. For evaluation of nutritional potential of some sub products, total phenol content (Folin-Ciocalteu reagent method), antioxidant activity (DPPH method), and calcium, magnesium, sodium, potassium and phosphorus content (ICP-AES) were determined in 'Sweetheart' cherry peel. Results were compared with those of 'Tulameen' raspberry fruit cultivated in the same region. Raspberry is recognised to having a high level of antioxidants, minerals and fibre. Average concentrations of 2793.8 mg gallic acid equivalent kg-1, 3450.9 mg TE kg-1, (Ca) 317.7, (Mg) 267.5, (Na) 9.8, (K) 2196.2, and (P) 407.5 mg kg-1 were found in 'Sweetheart' cherry peel. Regarding raspberries, concentrations of 1411.8 mg gallic acid equivalent kg-1, 2709.5 mg TE kg-1, (Ca) 281.9, (Mg) 227.2, (Na) 1.3, (K) 1646.7, and (P) 76.7 mg kg-1 were found. All nutritional concentrations in peel were significantly higher than in the remaining fruit, showing its potential for nutrition complement production.
- Major and minor minerals of ‘Sweetheart’ cherry stone from Cova da BeiraPublication . Antunes, Paulo; Pintado, Cristina Miguel; Paulo, LuísaNowadays customers demand objective and trustworthy information about the geographical origin of agricultural products. The mineral composition of agricultural products and its relation with their terroir has been shown to be a useful tool in differentiating commodities produced in a limited region and subjected to certain quality requirements. Cereja da Cova da Beira is the legal term used to denominate cherries produced in the Cova da Beira region (PGI - protected geographical indication) at central eastern part of Portugal, since 1996. In order to establish a methodology to certify its origin, the mineral profile of 'Sweetheart' cherry stone from Cereja da Cova da Beira , was studied. Quantification of major (Ca, Mg, K, P and Na) and minor elements (Cu, Fe Mn and Zn) of stones was carried out by inductively coupled plasma atomic emission spectrometry (ICP-AES). The results showed that, among major elements, Ca was the predominant mineral (1424.62 mg kg-1, dry weight), followed by K and Mg, presenting similar concentrations (1092.17 and 987.55 mg kg-1, respectively), P (271.10 mg kg-1), and Na (24.71 mg kg-1). Regarding micro elements, Mn showed the highest mean value (19.71 mg kg-1), followed by Fe (17.59 mg kg-1), and Cu and Zn with similar levels (7.61 and 6.73 mg kg-1, respectively). On the basis of chemometric pattern recognition procedures, the mineral content of agricultural products and its relation with their area of production can be a useful tool in differentiating commodities produced in a limited region and subjected to certain quality requirements.
- Metal contaminants in Largemouth bass (Micropterus salmoides) collected in large dams from Tejo River basin and small irrigation damsPublication . Andrade, L.P.; Antunes, Paulo; Paulo, Luísa; Pereira, M.E.; Rodrigues, A.M.Largemouth bass (Micropterus salmoides) is a very important fresh water fish in the Portuguese regional cuisine mainly in the countryside (Central region and north Alentejo). Because there’s no aquaculture industry, all eaten largemouth bass in Portugal are collected in large dams (Basins of Tejo and Guadiana rivers) and small irrigation dams. For decades, the Tejo River received environmental pollutants from non-point and point sources that included intensive agriculture, industrial entities, municipalities and nuclear power plant. The aim of this work was to evaluate some metals (Cd, Cr, Cu, Fe, Hg, Mn, Pb, Zn) present on largemouth bass muscle tissue collected in the section of Tejo River that makes border between Portugal and Spain (TR) (N=9) and collected in three irrigation reservoirs (IR) located near Castelo Branco – Portugal (N=11). Individual were weighted and measured. Age was determined by examining fish scales, and sex determined by gonads observation. Samples for liver, dorsolateral muscle and tail muscle were collected from the right side of the fish. TR average weight 435.14g (±109.15), average length 278.33mm (±23.28), average K condition factor 1.98 (±0.09) and average age 3.11 years (±0,78) were similar (P>0.05) to IR average weight 410.84g (±137.71), average length 278.36mm (±31.13), average K condition factor 1.86 (±0.17) and average age 3.18 years (±0,60). Total mercury was determined in freeze-dried samples by atomic absorption spectrometry with thermal decomposition and gold amalgamation. For the other metal analysis, freeze-dried powders were mineralized with a mixture of HNO3 and H2O2, followed by ICP-OES quantification. Cd and Pb presented concentrations below LOQ (0.025 and 0.15mg.kg-1wet.weight, respectively) for all muscle samples. These values are below legal limits in EU (0.05 and 0.3mg.kg-1wet.weight, respectively). Average Hg levels are below legal limits (0.5mg.kg-1wet.weight) and muscle presented higher levels than liver. These contaminant levels indicate that are no contamination sources in the sampling sites. Cu, Cr, Fe, Mn and Zn are essential micronutrients. Their concentrations were not significantly different between dorsal and tail muscle and were higher in liver (P>0.05), with mean concentrations on muscle being, respectively: TR 0.16 (±0.07),